ArticleBio-protocol2018
Use of Gas Chromatography to Quantify Short Chain Fatty Acids in the Serum, Colonic Luminal Content and Feces of mice.
Article in Bio-protocol, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 44 citations in OpenAlex.
- Trial
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- The possible effects of folic acid and postbiotic yeast on doxorubicin-induced cardiotoxicity in rats: interactions among the gut microbiota, antioxidants, and NLrp3 activities.Journal of molecular histology · 2026Article
- A multi-tiered workflow for examining organic acid profiles delineates tissue-specific changes in fatty acyl partitioning during aging.Cell reports methods · 2026Article
- Phytosynbiotic Containing Double-Layer MicroencapsulatedAnimals : an open access journal from MDPI · 2026Article
- Milk Yield and Quality, Metabolic Profile and Oxidative Status in Lactating Goats, as Affected by Silage Based on Agro-Industrial By-Products.Animals : an open access journal from MDPI · 2026Article
- Dietary pulse prebiotic fibre intake in a rat obese pregnancy model alters maternal caecal microbiome and protects against steatosis in newly weaned offspring.Journal of nutritional science · 2026Article
- In Vitro Three-Step Technique Assessment of a Microencapsulated Phytosynbiotic from Yanang (Veterinary sciences · 2025Article
- Synbiotic delivery of arabinoxylan and a human-derived arabinoxylan-fermenting consortium influence mouse gut microbiome composition, metabolism, and resilience in sex-dependent ways.Food research international (Ottawa, Ont.) · 2025Article
- Antioxidative Effects of Defatted Rice Bran in Rats on AOM/DSS-Induced Colon Oxidative Stress and Correlations Between Gut Microbiota and Antioxidant Biomarkers.Food science & nutrition · 2025Article
- Probiotic Administration Contributes to the Improvement in Intestinal Dysregulation Induced by Allergic Contact Dermatitis.Microorganisms · 2025Article
- Effects of butyrate glyceride-based feed ingredient supplementation on weanling pig performance, fecal dry matter, serum chemistry, and intestinal short chain fatty acid concentration.Journal of animal science · 2025Article
- Tailoring the natural rare sugars D-tagatose and L-sorbose to produce novel functional carbohydrates.NPJ science of food · 2024Article
- Impact of Gut Microbiota and SCFAs in the Pathogenesis of PCOS and the Effect of Metformin Therapy.International journal of molecular sciences · 2024Article
- A Chiral-LC-MS Method for the Simultaneous Quantification of Short-Chain Fatty Acids and D/L-Lactate in the Ruminal Fluid of Dairy Cows.Molecules (Basel, Switzerland) · 2024Article
- Approach to the diagnosis and management of dysbiosis.Frontiers in nutrition · 2024Review
- Effects of Mimosa caesalpiniifolia pre-formulation on the intestinal barrier during sodium dextran sulfate-induced colitis in Wistar ratsBiomedica : revista del Instituto Nacional de Salud · 2023Article
- Role of Dietary Defatted Rice Bran in the Modulation of Gut Microbiota in AOM/DSS-Induced Colitis-Associated Colorectal Cancer Rat Model.Nutrients · 2023Article
- Gut microbiota short-chain fatty acids and their impact on the host thyroid function and diseases.Frontiers in endocrinology · 2023Review
- Effects of kefir fermented milk beverage on sodium dextran sulfate (DSS)-induced colitis in rats.Heliyon · 2023Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Short-Chain Fatty Acids (SCFAs) are a product of the fermentation of resistant starches and dietary fibers by the gut microbiota. The most important SCFA are acetate (C2), propionate (C3) and butyrate (C4). These metabolites are formed and absorbed in the colon and then transported through the hepatic vein to the liver. SCFAs are more concentrated in the intestinal lumen than in the serum. Butyrate is largely consumed in the gut epithelium, propionate in the liver and acetate in the periphery. SCFAs act on many cells including components of the immune system and epithelial cells by two main mechanisms: activation of G-protein coupled receptors (GPCRs) and inhibition of histone deacetylase. Considering the association between changes in SCFA concentrations and the development of diseases, methods to quantify these acids in different biological samples are important. In this study, we describe a protocol using gas chromatography to quantify SCFAs in the serum, feces and colonic luminal content. Separation of compounds was performed using a DB-23 column (60 m x 0.25 mm internal diameter [
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.