ArticleBio-protocol2018
Extraction and Analysis of Bacterial Teichoic Acids.
Article in Bio-protocol, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Article
- Fermentation-Dependent Cell Wall-Associated Immunostimulation in a Red Pepper-BasedFoods (Basel, Switzerland) · 2026Article
- Human serum triglycerides promote Staphylococcus aureus biofilm formation and antibiotic tolerance.NPJ biofilms and microbiomes · 2026Article
- Article
- A teichoic acid-like wall modification associated with immune suppression is socially regulated inmBio · 2026Article
- Methicillin-resistantAntimicrobial agents and chemotherapy · 2026Article
- Article
- Article
- A teichoic acid-like wall modification associated with immune suppression is socially regulated inbioRxiv : the preprint server for biology · 2026Article
- Human neutrophil α-defensin HNP1 interacts with bacterial cell wall components to promote biofilm formation byBiofilm · 2025Article
- ThemBio · 2025Article
- Optimization of the Purification Process forJournal of microbiology and biotechnology · 2025Article
- A broad-spectrum antibiotic targets multiple-drug-resistant bacteria with dual binding targets and no detectable resistance.Nature communications · 2025Article
- D-Serine Can Modify the Wall Teichoic Acid of MRSA via theInternational journal of molecular sciences · 2025Article
- MprF-mediated immune evasion is necessary for Lactiplantibacillus plantarum resilience in the Drosophila gut during inflammation.PLoS pathogens · 2024Article
- Lipoteichoic acids influence cell shape and bacterial division of Streptococcus suis serotype 2, but play a limited role in the pathogenesis of the infection.Veterinary research · 2024Article
- Article
- Extensive re-modelling of the cell wall during the development ofbioRxiv : the preprint server for biology · 2023Article
- Wall Teichoic Acids Facilitate the Release of Toxins from the Surface of Staphylococcus aureus.Microbiology spectrum · 2022Article
- The Role of β-Glycosylated Wall Teichoic Acids in the Reduction of Vancomycin Susceptibility in Vancomycin-Intermediate Staphylococcus aureus.Microbiology spectrum · 2021Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Teichoic acids (TA) are anionic polymers comprised of polyol phosphate repeat units that are abundant in the cell wall of Gram-positive bacteria. Both wall teichoic acid (WTA) and lipoteichoic acid (LTA) play important roles in regulating cell wall remodeling as well as conferring antibiotic resistance. To analyze TA, we describe a polyacrylamide gel electrophoresis (PAGE) method for both WTA and LTA. To extract crude WTA, the peptidoglycan sacculus is first isolated and WTA is then liberated by hydrolysis. LTA is extracted by 1-butanol and pre-treated with lipase to prevent aggregation and improve single-band resolution by PAGE. Crude extracts of both TAs are then subjected to PAGE followed by Alcian blue and silver staining. These protocols are easily adoptable by laboratories interested in rapidly analyzing TAs and can be used determine the relative abundance, relative polymer length and whether TAs are glycosylated. More detailed TA structural and compositional information can be obtained using the described purification protocols by nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS) analysis.
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