ArticleAnnals of translational medicine2021
Involvement of ferroptosis in the granulosa cells proliferation of PCOS through the circRHBG/miR-515/SLC7A11 axis.
Article in Annals of translational medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 62 citations in OpenAlex.
- Bushen Huatan Formula Ameliorates Early Chronic Low-Grade Inflammation in Obese PCOS Via the TLR4/NF-κB Signaling Pathway: An Integrated Network Pharmacology and Experimental Study.Immunity, inflammation and disease · 2026Article
- Interconnected cell death pathways: central mechanisms and therapeutic targets in impaired follicular development of polycystic ovary syndrome.Journal of ovarian research · 2026Review
- Mitochondria and polycystic ovary syndrome: The role of ferroptosis, inflammasomes, and endoplasmic reticulum stress.International journal of biological sciences · 2026Review
- Circular RNA expression profiling and the potential role of hsa_circ_0005379 in decreased ovarian reserve.Experimental and therapeutic medicine · 2025Article
- Global profiling of protein lactylome in porcine granulosa cells.Journal of ovarian research · 2025Article
- The Role of Ferroptosis in Women's Health and Diseases.MedComm · 2025Review
- hnRNPA2B1 restrains granulosa cell ferroptosis by mJournal of ovarian research · 2025Article
- The Role of Ovarian Granulosa Cells Related-ncRNAs in Ovarian Dysfunctions: Mechanism Research and Clinical Exploration.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Mechanisms of Granulosa Cell Programmed Cell Death and Follicular Atresia in Polycystic Ovary Syndrome.Physiological research · 2025Review
- Whole transcriptome analysis and construction of gene regulatory networks of granulosa cells from patients with polycystic ovary syndrome (PCOS).European journal of medical research · 2025Article
- The pathogenesis, therapeutic targets and drugs of polycystic ovary syndrome.Frontiers in endocrinology · 2025Review
- Mechanisms of follicular atresia: focus on apoptosis, autophagy, and ferroptosis.Frontiers in endocrinology · 2025Review
- Effects of Trace Elements and Vitamins on the Synthesis of Steroid Hormones in Follicular Granulosa Cells of Yak.Veterinary sciences · 2024Article
- METTL3 silencing inhibits ferroptosis to suppress ovarian fibrosis in PCOS by upregulating m6A modification of GPX4.Journal of molecular histology · 2024Article
- Biomarker potential of competing endogenous RNA networks in Polycystic Ovary Syndrome (PCOS).Non-coding RNA research · 2024Review
- Role of circular RNA/miRNA axes in the pathophysiology of polycystic ovary syndrome.Molecular biology reports · 2024Review
- A matter of new life and cell death: programmed cell death in the mammalian ovary.Journal of biomedical science · 2024Review
- The Role of MicroRNA, Long Non-Coding RNA and Circular RNA in the Pathogenesis of Polycystic Ovary Syndrome: A Literature Review.International journal of molecular sciences · 2024Review
- Ovarian ferroptosis induced by androgen is involved in pathogenesis of PCOS.Human reproduction open · 2024Article
- Broadening horizons: the role of ferroptosis in polycystic ovary syndrome.Frontiers in endocrinology · 2024Review
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPolycystic ovary syndrome (PCOS) is the most common endocrine disease among women, but its etiology remains unknown. In recent years, many circular RNAs (circRNAs) have been confirmed to be related to the development of PCOS. The role and mechanism of circRNA in the development of PCOS need to be further explored.
methodsIn the present study, we used the circRNA chip to detect the difference in the expression of circRNA in the granulosa cells of PCOS patients and controls. Five upregulated circRNAs were then selected for quantitative reverse transcription polymerase chain reaction (qRT-PCR) verification, and circRHBG was found to be upregulated in PCOS. Subsequently, Cell Counting Kit-8 assay and EdU assay were used to observe the effect of circRHBG on the proliferation of KGN and SVOG cells. Furthermore, the pairwise binding relationship between circRHBG/miR515-5 and miR515-5p/SLC7A11 was verified by luciferase reporter assay. The interaction between circRHBG and SLC7A11 was detected with qRT-PCR and Western blot.
resultsCircRNA high-throughput chips and qRT-PCR verified that circRHBG was significantly upregulated in granular cells of PCOS patients. Knockdown of circRHBG inhibits KGN and SVOG cell proliferation. Luciferase reporter assays and Ago2-RIP detection showed that circRHBG competes with SLC7A11 to bind to miR-515-5p. Subsequent experiments verified knockdown of circRHBG promotes ferroptosis in PCOS.
conclusionscircRHBG inhibits ferroptosis in PCOS cells through the circRHBG/miR-515-5p/SLC7A11 axis in PCOS, which may provide new diagnostic molecular markers and therapeutic targets for PCOS.
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