ArticleScientific reports2021
Engineering an efficient and bright split Corynactis californica green fluorescent protein.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Engineering a new tripartite split-ccGFP system from Corynactis californica for detecting protein-protein interactions.Scientific reports · 2025Article
- Protocol for engineering poly(ethylene terephthalate) hydrolases via directed evolution using a high-throughput screening assay.STAR protocols · 2025Article
- Engineering PHL7 for improved poly(ethylene terephthalate) depolymerization via rational design and directed evolution.Chem catalysis · 2025Article
- A High-Throughput Screening Platform for Engineering Poly(ethylene Terephthalate) Hydrolases.ACS catalysis · 2024Article
- Engineering highly stable variants of Corynactis californica green fluorescent proteins.Protein science : a publication of the Protein Society · 2024Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Split green fluorescent protein (GFP) has been used in a panoply of cellular biology applications to study protein translocation, monitor protein solubility and aggregation, detect protein-protein interactions, enhance protein crystallization, and even map neuron contacts. Recent work shows the utility of split fluorescent proteins for large scale labeling of proteins in cells using CRISPR, but sets of efficient split fluorescent proteins that do not cross-react are needed for multiplexing experiments. We present a new monomeric split green fluorescent protein (ccGFP) engineered from a tetrameric GFP found in Corynactis californica, a bright red colonial anthozoan similar to sea anemones and scleractinian stony corals. Split ccGFP from C. californica complements up to threefold faster compared to the original Aequorea victoria split GFP and enable multiplexed labeling with existing A. victoria split YFP and CFP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.