ArticleScientific reports2021
Development of CIDEA reporter mouse model and its application for screening thermogenic drugs.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Thermoregulatory adaptations to cold in C3H/HeJ mice are independent of ADRB3 signaling.Scientific reports · 2026Article
- Nonanoic acid and cholecystokinin induce beige adipogenesis.Food science and biotechnology · 2025Article
- Mouse vascularized adipose spheroids: an organotypic model for thermogenic adipocytes.Frontiers in endocrinology · 2024Article
- miR-889-3p Facilitates the Browning Process of White Adipocyte Precursors by Targeting theInternational journal of molecular sciences · 2023Article
- Use of CIDEA Reporter Mouse Model for Screening Thermogenic Fat-Activating Drugs.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Anti-obesity effects of the dual-active adenosine AInternational journal of obesity (2005) · 2022Article
- Bioluminescence imaging of mouse monocyte chemoattractant protein-1 expression in inflammatory processes.Acta biochimica et biophysica Sinica · 2022Article
- Anti-obesity effects of heat-transformed green tea extract through the activation of adipose tissue thermogenesis.Nutrition & metabolism · 2022Article
- Application and trend of bioluminescence imaging in metabolic syndrome research.Frontiers in chemistry · 2022Review
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell death-inducing DNA fragmentation factor-like effector A (CIDEA) is a lipid droplet-associated protein and is a known marker of the thermogenic capacity of brown/beige adipocytes. To monitor the expression of CIDEA in live mice in a non-invasive manner, we generated CIDEA reporter mice expressing multicistronic mRNAs encoding CIDEA, luciferase 2, and tdTomato proteins under the control of the Cidea promoter. The expression level of endogenous CIDEA protein in adipose tissue was not affected by the expression of polycistronic reporters. The two CIDEA reporters, luciferase 2 and tdTomato, correctly reflected CIDEA protein levels. Importantly, luciferase activity was induced by cold exposure and the treatment with β3-adrenergic receptor agonist CL316,243 in interscapular and inguinal adipose tissue, which was detectable by in vivo bioluminescence imaging. We further evaluated the effects of candidate brown adipogenic agents using this CIDEA reporter system and demonstrated a positive correlation between drug-induced luciferase activity and thermogenic gene expression levels both in vitro and in vivo. Collectively, we established a dual CIDEA reporter mouse model in which fluorescence and luminescence signals correctly reflect CIDEA expression, and therefore, suggested that this reporter system can be used to evaluate the thermogenic efficacy of candidate molecules.
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