Evidence map›Paper›PMID 34528139›Full record

ArticleCellular and molecular neurobiology2022

Multiomic Analyses of Dopaminergic Neurons Isolated from Human Substantia Nigra in Parkinson's Disease: A Descriptive and Exploratory Study.

Affif Zaccaria, Paola Antinori, Virginie Licker, Enikö Kövari, Johannes A Lobrinus, Pierre R Burkhard

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In one paragraph

Article in Cellular and molecular neurobiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  12. New Multiomic Studies Shed Light on Cellular Diversity and Neuronal Susceptibility in Parkinson's Disease.Movement disorders : official journal of the Movement Disorder Society · 2025
    Review
  13. Article
  14. Proteomic insights into the biology of dopaminergic neurons.Frontiers in molecular neuroscience · 2025
    Review
  15. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Affif Zaccaria *Neuroproteomics Group, University Medical Center, Faculty of Medicine, Geneva University, Geneva, Switzerland. Affif.zaccaria@unige.ch.ORCID http://orcid.org/0000-0001-9623-6269
Paola Antinori *Neuroproteomics Group, University Medical Center, Faculty of Medicine, Geneva University, Geneva, Switzerland.
Virginie LickerNeuroproteomics Group, University Medical Center, Faculty of Medicine, Geneva University, Geneva, Switzerland.
Enikö KövariDepartment of Psychiatry, Geneva University Hospitals, Geneva, Switzerland.
Johannes A LobrinusDepartment of Pathology, Geneva University Hospitals, Geneva, Switzerland.
Pierre R BurkhardNeuroproteomics Group, University Medical Center, Faculty of Medicine, Geneva University, Geneva, Switzerland.

Funding

Swiss National Foundation (CH) 31003A_143987
6 · The paper itself

Abstract

Dopaminergic neurons (DA) of the substantia nigra pars compacta (SNpc) selectively and progressively degenerate in Parkinson's disease (PD). Until now, molecular analyses of DA in PD have been limited to genomic or transcriptomic approaches, whereas, to the best of our knowledge, no proteomic or combined multiomic study examining the protein profile of these neurons is currently available. In this exploratory study, we used laser capture microdissection to extract regions from DA in 10 human SNpc obtained at autopsy in PD patients and control subjects. Extracted RNA and proteins were identified by RNA sequencing and nanoliquid chromatography-mass spectrometry, respectively, and the differential expression between PD and control group was assessed. Qualitative analyses confirmed that the microdissection protocol preserves the integrity of our samples and offers access to specific molecular pathways. This multiomic analysis highlighted differential expression of 52 genes and 33 proteins, including molecules of interest already known to be dysregulated in PD, such as LRP2, PNMT, CXCR4, MAOA and CBLN1 genes, or the Aldehyde dehydrogenase 1 protein. On the other hand, despite the same samples were used for both analyses, correlation between RNA and protein expression was low, as exemplified by the CST3 gene encoding for the cystatin C protein. This is the first exploratory study analyzing both gene and protein expression of laser-dissected neuronal parts from SNpc in PD. Data are available via ProteomeXchange with identifier PXD024748 and via GEO with identifier GSE 169755.

Indexed as

Dopaminergic NeuronsParkinson DiseaseAldehyde Dehydrogenase 1 FamilyCystatin CHumansRNASubstantia NigraAldehyde Dehydrogenase 1 FamilyCystatin CRNADopaminergic neuronsHuman brain tissueLaser capture microdissectionParkinson’s diseaseProteomicsTranscriptomics

Identifiers

PMID34528139
PMCPMC9561004

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.