ArticleProceedings of the National Academy of Sciences of the United States of America1978
Molecular cytochemistry: incorporation of fluorescently labeled actin into living cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1978. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 149 citations in OpenAlex.
- Neutrophil-like HL-60 cells expressing only GFP-tagged β-actin exhibit nearly normal motility.Cytoskeleton (Hoboken, N.J.) · 2020Article
- Mechanisms and in vivo functions of contact inhibition of locomotion.Nature reviews. Molecular cell biology · 2017Review
- From single molecules to life: microscopy at the nanoscale.Analytical and bioanalytical chemistry · 2016Review
- Fluorescent protein biosensors applied to microphysiological systems.Experimental biology and medicine (Maywood, N.J.) · 2015Review
- Thermodynamics and kinetics of DNA nanotube polymerization from single-filament measurements.Chemical science · 2015Article
- Optimized delivery of fluorescently labeled proteins in live bacteria using electroporation.Histochemistry and cell biology · 2014Article
- Genetically encoded molecular probes to visualize and perturb signaling dynamics in living biological systems.Journal of cell science · 2014Review
- Long-lived intracellular single-molecule fluorescence using electroporated molecules.Biophysical journal · 2013Article
- The binding of fluorophores to proteins depends on the cellular environment.Angewandte Chemie (International ed. in English) · 2011Article
- Reporting from the field: genetically encoded fluorescent reporters uncover signaling dynamics in living biological systems.Annual review of biochemistry · 2011Review
- Visualizing biochemical activities in living cells.Nature chemical biology · 2009Article
- Microtubules and microscopes: how the development of light microscopic imaging technologies has contributed to discoveries about microtubule dynamics in living cells.Molecular biology of the cell · 1998Review
- Transfer of macromolecules into living adult cardiomyocytes by microinjection.Molecular and cellular biochemistry · 1997Article
- Myosin II transport, organization, and phosphorylation: evidence for cortical flow/solation-contraction coupling during cytokinesis and cell locomotion.Molecular biology of the cell · 1996Article
- Interpreting photoactivated fluorescence microscopy measurements of steady-state actin dynamics.Biophysical journal · 1995Article
- 1,N6-etheno deoxy and ribo adenosine and 3,N4-etheno deoxy and ribo cytidine phosphoramidites. Strongly fluorescent structures for selective introduction in defined sequence DNA and RNA molecules.Nucleic acids research · 1994Article
- Fluorescent actin analogs with a high affinity for profilin in vitro exhibit an enhanced gradient of assembly in living cells.The Journal of cell biology · 1994Article
- Fluorescence anisotropy imaging microscopy maps calmodulin binding during cellular contraction and locomotion.The Journal of cell biology · 1993Article
- Control of actin polymerization in live and permeabilized fibroblasts.The Journal of cell biology · 1991Article
- Keratin incorporation into intermediate filament networks is a rapid process.The Journal of cell biology · 1991Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin labeled with 5-iodoacetamidofluorescein has been incorporated into the functional pool of actin in Chaos carolinensis and Physarum polycephalum by direct microinjection. The functional activity of the labeled actin has been analyzed at three levels of organization as: (a) with the purified actin, (b) in motile extracts of cells, and (c) in living motile cells. The labeled actin exhibited normal polymerization and activated myosin ATPase to a similar extent as unlabeled controls. Labeled actin and endogenous actin were incorporated into contracted pellets to approximately the same extent in motile cell extracts. After labeled actin had been microinjected into single C. carolinensis cells, the fluorescent actin spread into both the endoplasm and etoplasm without forming distinct fibrils. In contrast, fluorescent bundles developed in the ectoplasm of P. polycephalum following microinjection of labeled actin. This experimental method in conjunction with fluorescence spectroscopic techniques could become a powerful tool for studying the intracellular distribution and structural changes of components in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.