Evidence map›Paper›PMID 34527795›Full record

ArticleData in brief2021

(Phospho)Proteomic dataset of ischemia- and ultrasound- stimulated mouse cardiac endothelial cells in vitro.

Uchenna Emechebe, David Giraud, Azzdine Y Ammi, Kristin L Scott, Jon M Jacobs, Jason E McDermott, Igor V Dykan, Nabil J Alkayed, Anthony P Barnes, Sanjiv Kaul and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Data in brief, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Uchenna EmechebeKnight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, 97239, USA.
David GiraudKnight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, 97239, USA.
Azzdine Y AmmiKnight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, 97239, USA.
Kristin L ScottDepartment of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, OR, 97239, USA.
Jon M JacobsBiological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99354, USA.
Jason E McDermottBiological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99354, USA.
Igor V DykanKnight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, 97239, USA.
Nabil J AlkayedKnight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, 97239, USA.
Anthony P BarnesKnight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, 97239, USA.
Sanjiv KaulKnight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, 97239, USA.
Catherine M DavisKnight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, 97239, USA.

Funding

Equipment Supplement Request for a Research Resource for Ultra-sensitive High Throughput ProteomicsP41GM103493 · NIGMS · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI SMITH, RICHARD D · 2012 to 2022
$22.6M
NIGMS NIH HHS P41 GM103493
6 · The paper itself

Abstract

Cardiac endothelial cells respond to both ischemia and therapeutic ultrasound; the proteomic changes underlying these responses are unknown. This data article provides raw and processed data resulting from our global, unbiased phosphoproteomics investigation conducted on primary mouse cardiac endothelial cells exposed to ischemia (2-hour oxygen glucose deprivation) and ultrasound (250 kHz, 1.2 MPa) in vitro [1]. Proteins were extracted from cell lysates and enriched phosphopeptides were analyzed with a high mass accuracy liquid chromatrography (LC) - tandem mass spectrometry (MS/MS) proteomic platform, yielding multiple alterations in both total protein levels and phosphorylation events in response to ischemic injury and ultrasound. This dataset can be used as a reference for future studies on the cardiac endothelial response to ischemia and the mechanistic underpinnings of the cellular response to ultrasound, with the potential to yield clinically relevant therapeutic targets.

Indexed as

Endothelial cellHeartIschemiaPhosphoproteomicsProteomicsUltrasound

Identifiers

PMID34527795
PMCPMC8429095

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.