Evidence map›Paper›PMID 34527719›Full record

ArticleMicrobial cell (Graz, Austria)2021

The long and winding road of reverse genetics in

Miguel A Chiurillo, Noelia Lander

Open access · goldAbstract readEditorial
In one paragraph

Article in Microbial cell (Graz, Austria), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 14 citations in OpenAlex.

  1. New hit compounds againstAntimicrobial agents and chemotherapy · 2026
    Article
  2. Article
  3. Cloning-Free Genome Editing by CRISPR/T7RNAP/Cas9 in Trypanosoma cruzi.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  4. A limitation lifted: A conditional knockdown system reveals essential roles for Polo-like kinase and Aurora kinase 1 inProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  5. Article
  6. Review
  7. Genome deletions to overcome the directed loss of gene function inFrontiers in cellular and infection microbiology · 2022
    Article
  8. Ablation of theFrontiers in cellular and infection microbiology · 2022
    Article
  9. Trypanin Disruption Affects the Motility and Infectivity of the ProtozoanFrontiers in cellular and infection microbiology · 2021
    Article
  10. Gene editing of putative cAMP and CaThe Journal of eukaryotic microbiology
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Miguel A ChiurilloDepartment of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA.
Noelia LanderDepartment of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA.
University of Cincinnati · US

Funding

Large-scale CRISPR screening of essential calcium-related genes in the human parasite Tr. cruziR00AI137322 · NIAID · UNIVERSITY OF CINCINNATI · PI LANDER, NOELIA · 2021 to 2022
$498k
Large-scale CRISPR screening of essential calcium-related genes in the human parasite Trypanosoma cruziK99AI137322 · NIAID · UNIVERSITY OF GEORGIA · PI LANDER, NOELIA · 2019 to 2020
$209k
NIAID NIH HHS K99 AI137322NIAID NIH HHS R00 AI137322
6 · The paper itself

Abstract

Trypanosomes are early divergent protists with distinctive features among eukaryotic cells. Together with

Indexed as

Chagas diseaseCRISPR/Cas9gene knock-ingene knockoutgene tagginggenome editingtrypanosomes

Identifiers

PMID34527719
PMCPMC8404153
OpenAlexW3196583908

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.