ArticlePeerJ2021
Issues of under-representation in quantitative DNA metabarcoding weaken the inference about diet of the tundra vole
Article in PeerJ, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- Dietary DNA Metabarcoding From Animal Fecal Samples.Current protocols · 2026Article
- Article
- Ecological Drivers of Molt-Breeding Overlap, an Unusual Life-History Strategy of Small-Island Birds?Ecology and evolution · 2025Article
- Is the diet cyclic phase-dependent in boreal vole populations?Ecology and evolution · 2024Article
- Low Genetic Variability of the Tundra Vole in Lithuania.Animals : an open access journal from MDPI · 2024Article
- Herbivorous dietary selection shown by hawfinch (Royal Society open science · 2023Article
- DNA High-Throughput Sequencing for Arthropod Gut Content Analysis to Evaluate Effectiveness and Safety of Biological Control Agents.Neotropical entomology · 2023Review
- Small rodent monitoring at Birkebeiner Road, Norway.Biodiversity data journal · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the last decade, methods based on high-throughput sequencing such as DNA metabarcoding have opened up for a range of new questions in animal dietary studies. One of the major advantages of dietary metabarcoding resides in the potential to infer a quantitative relationship between sequence read proportions and biomass of ingested food. However, this relationship's robustness is highly dependent on the system under study, calling for case-specific assessments. Herbivorous small rodents often play important roles in the ecosystem, and the use of DNA metabarcoding for analyses of rodent diets is increasing. However, there has been no direct validation of the quantitative reliability of DNA metabarcoding for small rodents. Therefore, we used an experimental approach to assess the relationship between input plant biomass and sequence reads proportions from DNA metabarcoding in the tundra vole
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Registered trials
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