Evidence map›Paper›PMID 34526658›Full record

ArticleCommunications biology2021

EndoBind detects endogenous protein-protein interactions in real time.

Anke Bill, Sheryll Espinola, Daniel Guthy, Jacob R Haling, Mylene Lanter, Min Lu, Anthony Marelli, Angelica Mendiola, Loren Miraglia, Brandon L Taylor and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Protein Engineering for Spatiotemporally Resolved Cellular Monitoring.Annual review of analytical chemistry (Palo Alto, Calif.) · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Anke BillNovartis Institute for Biochemical Research, Oncology, Cambridge, MA, USA. anke.bill@novartis.com.ORCID 0000-0003-1244-7574
Sheryll EspinolaGenomics Institute of the Novartis Research Foundation, Assay Development and High Throughput Screening, San Diego, CA, USA.
Daniel GuthyNovartis Institute for Biochemical Research, Oncology, Basel, Switzerland.
Jacob R HalingGenomics Institute of the Novartis Research Foundation, Assay Development and High Throughput Screening, San Diego, CA, USA.
Mylene LanterNovartis Institute for Biochemical Research, Oncology, Basel, Switzerland.
Min LuGenomics Institute of the Novartis Research Foundation, Assay Development and High Throughput Screening, San Diego, CA, USA.
Anthony MarelliGenomics Institute of the Novartis Research Foundation, Assay Development and High Throughput Screening, San Diego, CA, USA.
Angelica MendiolaGenomics Institute of the Novartis Research Foundation, Assay Development and High Throughput Screening, San Diego, CA, USA.
Loren MiragliaGenomics Institute of the Novartis Research Foundation, Assay Development and High Throughput Screening, San Diego, CA, USA.ORCID 0000-0003-2018-628X
Brandon L TaylorGenomics Institute of the Novartis Research Foundation, Assay Development and High Throughput Screening, San Diego, CA, USA.
Leonardo VargasGenomics Institute of the Novartis Research Foundation, Assay Development and High Throughput Screening, San Diego, CA, USA.
Anthony P OrthGenomics Institute of the Novartis Research Foundation, Assay Development and High Throughput Screening, San Diego, CA, USA.
Frederick J KingGenomics Institute of the Novartis Research Foundation, Assay Development and High Throughput Screening, San Diego, CA, USA. fred.king@novartis.com.ORCID 0000-0001-5958-5451

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present two high-throughput compatible methods to detect the interaction of ectopically expressed (RT-Bind) or endogenously tagged (EndoBind) proteins of interest. Both approaches provide temporal evaluation of dimer formation over an extended duration. Using examples of the Nrf2-KEAP1 and the CRAF-KRAS-G12V interaction, we demonstrate that our method allows for the detection of signal for more than 2 days after substrate addition, allowing for continuous monitoring of endogenous protein-protein interactions in real time.

Indexed as

HEK293 CellsHigh-Throughput Screening AssaysHumansKelch-Like ECH-Associated Protein 1NF-E2-Related Factor 2Protein BindingProto-Oncogene Proteins p21(ras)KEAP1 protein, humanKelch-Like ECH-Associated Protein 1KRAS protein, humanNFE2L2 protein, humanNF-E2-Related Factor 2Proto-Oncogene Proteins p21(ras)

Identifiers

PMID34526658
PMCPMC8443649

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.