ArticleLight, science & applications2021
Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography.
Article in Light, science & applications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- Inverse-scattering of absorptive samples via beam propagation.bioRxiv : the preprint server for biology · 2026Article
- Label-free structural imaging of plant roots and microbes using third-harmonic generation microscopy.Scientific reports · 2025Article
- Recent Advances and Current Trends in Transmission Tomographic Diffraction Microscopy.Sensors (Basel, Switzerland) · 2024Review
- Beyond conventional microscopy: Observing kidney tissues by means of fourier ptychography.Frontiers in physiology · 2023Article
- Label-free optical interferometric microscopy to characterize morphodynamics in living plants.Frontiers in plant science · 2023Review
- Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry.Nature photonics · 2022Article
- Finding intracellular lipid droplets from the single-cell biolens' signature in a holographic flow-cytometry assay.Biomedical optics express · 2022Article
- Quantitative phase imaging through an ultra-thin lensless fiber endoscope.Light, science & applications · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-cell phase-contrast tomography promises to become decisive for studying 3D intracellular structures in biology. It involves probing cells with light at wide angles, which unfortunately requires complex systems. Here we show an intriguing concept based on an inherent natural process for plants biology, i.e., dehydration, allowing us to easily obtain 3D-tomography of onion-epidermal cells' nuclei. In fact, the loss of water reduces the turgor pressure and we recognize it induces significant rotation of cells' nuclei. Thanks to the holographic focusing flexibility and an ad-hoc angles' tracking algorithm, we combine different phase-contrast views of the nuclei to retrieve their 3D refractive index distribution. Nucleolus identification capability and a strategy for measuring morphology, dry mass, biovolume, and refractive index statistics are reported and discussed. This new concept could revolutionize the investigation in plant biology by enabling dynamic 3D quantitative and label-free analysis at sub-nuclear level using a conventional holographic setup.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.