ArticleCell reports2021
Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila.
Article in Cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Altering the dosage of meiotic crossover-associated RING finger proteins affects crossover number and interference in Drosophila.G3 (Bethesda, Md.) · 2026Article
- Immune surveillance interrupted: how cancer exploits trogocytosis.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2026Review
- Selection, counterselection, and conditional gene expression for genetic biocontrol of insects.Insect science · 2026Review
- Altering dosage of meiotic crossover-associated RING finger proteins affects crossover number and interference in Drosophila.bioRxiv : the preprint server for biology · 2026Article
- The Template-Jumping Editing Approach inInternational journal of molecular sciences · 2025Article
- CRISPR/Cas9-mediated gene targeting at BBM2 locus demonstrates HDR-assisted precise knock-in in banana cv. Grand Naine.Plant cell reports · 2025Article
- Differential elimination of marked sex chromosomes enables production of nontransgenic male mosquitoes in a single strain.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- GoldenBraid2.0Synthetic biology (Oxford, England) · 2025Article
- Diversity ofFrontiers in cell and developmental biology · 2025Article
- Redesigning the Drosophila histone gene cluster: an improved genetic platform for spatiotemporal manipulation of histone function.Genetics · 2024Article
- Expanding theeLife · 2024Article
- NDUFS4 regulates cristae remodeling in diabetic kidney disease.Nature communications · 2024Article
- split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- split-intein Gal4 provides intersectional genetic labeling that is fully repressible by Gal80.bioRxiv : the preprint server for biology · 2023Article
- Synthetic Assembly DNA Cloning to Build Plasmids for Multiplexed Transgenic Selection, Counterselection or Any Other Genetic Strategies Using Drosophila melanogaster.Current protocols · 2023Article
- Serial Recombineering Cloning to Build Selectable and Tagged Genomic P[acman] BAC Clones for Selection Transgenesis and Functional Gene Analysis using Drosophila melanogaster.Current protocols · 2023Article
- Multiplexed Transgenic Selection and Counterselection Strategies to Expedite Genetic Manipulation Workflows Using Drosophila melanogaster.Current protocols · 2023Article
- Genetic and viral approaches to record or manipulate neurons in insects.Current opinion in insect science · 2021Review
- Determining effective drug concentrations for selection and counterselection genetics inSTAR protocols · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The power of Drosophila melanogaster as a model system relies on tractable germline genetic manipulations. Despite Drosophila's expansive genetics toolbox, such manipulations are still accomplished one change at a time and depend predominantly on phenotypic screening. We describe a drug-based genetic platform consisting of four selection and two counterselection markers, eliminating the need to screen for modified progeny. These markers work reliably individually or in combination to produce specific genetic outcomes. We demonstrate three example applications of multiplexed drug-based genetics by generating (1) transgenic animals, expressing both components of binary overexpression systems in a single transgenesis step; (2) dual selectable and counterselectable balancer chromosomes; and (3) selectable, fluorescently tagged P[acman] bacterial artificial chromosome (BAC) strains. We perform immunoprecipitation followed by proteomic analysis on one tagged BAC line, demonstrating our platform's applicability to biological discovery. Lastly, we provide a plasmid library resource to facilitate custom transgene design and technology transfer to other model systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.