ArticlePlant biotechnology journal2022
Genome- and transcriptome-wide association studies reveal the genetic basis and the breeding history of seed glucosinolate content in Brassica napus.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 82 citations in OpenAlex.
- Transcriptome Analysis Reveals Intensity-Dependent Regulation of UV-B Radiation on Glucosinolate Biosynthesis in Rapeseed Leaves.Plants (Basel, Switzerland) · 2026Article
- Glucosinolates inInternational journal of molecular sciences · 2026Review
- BnaC03.SEIPIN1 improves seed oil content by increasing the number of lipid droplets in Brassica napus.BMC plant biology · 2026Article
- Dissecting the genetic architecture of seed-related traits in Brassica napus by integrating multi-omics analysis and VIS-NIR hyperspectral imaging.Genome biology · 2026Article
- Joint analysis of GWAS and TILLING reveals the negative role of GLABRA2 in controlling seed oil content in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Biodiversity and genetic improvement ofFrontiers in plant science · 2026Review
- Transport of secondary metabolites in plants: Mechanistic insights and transporter engineering for crop improvement.Plant communications · 2025Review
- High-level production of health-beneficial glucoraphanin by multiplex editing of AOP2 gene family in mustard.Plant biotechnology journal · 2025Article
- Integrating genome assembly, structural variation map construction and GWAS reveal the impact of SVs on agronomic traits of Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Unlocking genetic potential: a review of the role of CRISPR/Cas technologies in rapeseed improvement.Stress biology · 2025Review
- Deciphering the heterogeneous glucosinolates composition in leaves and seeds: strategies for developing Brassica napus genotypes with low seed glucosinolates content but high leaf glucosinolates content.Molecular horticulture · 2025Article
- Genetic and transcriptome analyses of the effect of genotype-by-environment interactions on Brassica napus seed oil content.The Plant cell · 2025Article
- Integrative omics analysis reveals the genetic basis of fatty acid composition in Brassica napus seeds.Genome biology · 2025Article
- Homoeolog expression divergence contributes to time of day changes in transcriptomic and glucosinolate responses to prolonged water limitation in Brassica napus.The Plant journal : for cell and molecular biology · 2025Article
- Exploring silique number in Brassica napus L.: Genetic and molecular advances for improving yield.Plant biotechnology journal · 2024Review
- Natural variation in BnaA07.MKK9 confers resistance to Sclerotinia stem rot in oilseed rape.Nature communications · 2024Article
- Breeding and biotechnology approaches to enhance the nutritional quality of rapeseed byproducts for sustainable alternative protein sources- a critical review.Frontiers in plant science · 2024Review
- Transcriptional engineering for value enhancement of oilseed crops: a forward perspective.Frontiers in genome editing · 2024Review
- Two-Component System Genes inInternational journal of molecular sciences · 2023Article
- Targeted editing of multiple homologues of GTR1 and GTR2 genes provides the ideal low-seed, high-leaf glucosinolate oilseed mustard with uncompromised defence and yield.Plant biotechnology journal · 2023Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A high content of seed glucosinolates and their degradation products imposes anti-nutritional effects on livestock; therefore, persistent efforts are made to reduce the seed GSL content to increase the commercial value of rapeseed meal. Here, we dissected the genetic structure of SGC by genome-wide association studies (GWAS) combined with transcriptome-wide association studies (TWAS). Fifteen reliable quantitative trait loci (QTLs) were identified to be associated with the reduced SGC in modern B. napus cultivars by GWAS. Analysis of the selection strength and haplotypes at these QTLs revealed that low SGC was predominantly generated by the co-selection of qGSL.A02.2, qGSL.C02.1, qGSL.A09.2, and qGSL.C09.1. Integration of the results from TWAS, comprehensive bioinformatics, and POCKET algorithm analyses indicated that BnaC02.GTR2 (BnaC02g42260D) is a candidate gene underlying qGSL.C02.1. Using CRISPR/Cas9-derived Bna.gtr2s knockout mutants, we experimentally verified that both BnaC02.GTR2 and its three paralogs positively regulate seed GSL accumulation but negatively regulated vegetative tissue GSL contents. In addition, we observed smaller seeds with higher seed oil content in these Bna.gtr2 mutants. Furthermore, both RNA-seq and correlation analyses suggested that Bna.GTR2s might play a comprehensive role in seed development, such as amino acid accumulation, GSL synthesis, sugar assimilation, and oil accumulation. This study unravels the breeding selection history of low-SGC improvement and provides new insights into the molecular function of Bna.GTR2s in both seed GSL accumulation and seed development in B. napus.
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