ArticleApplied and environmental microbiology2021
Targeted Gene Insertion and Replacement in the Basidiomycete Ganoderma lucidum by Inactivation of Nonhomologous End Joining Using CRISPR/Cas9.
Article in Applied and environmental microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Establishment of CRISPR/Cas9 Genome Editing inJournal of fungi (Basel, Switzerland) · 2026Article
- Wherefore the Magic? The Evolutionary Role of Psilocybin in Nature.Ecology and evolution · 2026Article
- Development of a CRISPR/Cas9 Genome Editing System in DikaryoticJournal of fungi (Basel, Switzerland) · 2026Article
- Review
- Recent developments in CRISPR/Cas9 genome editing research for edible fungiculture.Functional & integrative genomics · 2026Review
- Screening of Monokaryotic Strains ofJournal of fungi (Basel, Switzerland) · 2025Article
- Targeted insertion of heterogenous DNA using Cas9-gRNA ribonucleoprotein-mediated gene editing inBioengineered · 2025Article
- CRISPR/Cas9-mediated genome editing in Ganoderma lucidum: recent advances and biotechnological opportunities.World journal of microbiology & biotechnology · 2025Review
- Gene Editing inJournal of fungi (Basel, Switzerland) · 2025Review
- Towards engineering agaricomycete fungi for terpenoid production.Journal of industrial microbiology & biotechnology · 2024Review
- Effect of a Mating Type Gene Editing inJournal of fungi (Basel, Switzerland) · 2024Article
- Enhanced extracellular production of laccase in Coprinopsis cinerea by silencing chitinase gene.Applied microbiology and biotechnology · 2024Article
- Pleurotus ostreatus as a model mushroom in genetics, cell biology, and material sciences.Applied microbiology and biotechnology · 2024Review
- Utilization of CRISPR-Cas genome editing technology in filamentous fungi: function and advancement potentiality.Frontiers in microbiology · 2024Review
- The Cas9-gRNA ribonucleoprotein complex-mediated editing of pyrG in Ganoderma lucidum and unexpected insertion of contaminated DNA fragments.Scientific reports · 2023Article
- Application progress of CRISPR/Cas9 genome-editing technology in edible fungi.Frontiers in microbiology · 2023Review
- Multiplex gene precise editing and large DNA fragment deletion by the CRISPR-Cas9-TRAMA system in edible mushroom Cordyceps militaris.Microbial biotechnology · 2022Article
- A Simple and Efficient CRISPR/Cas9 System Using a Ribonucleoprotein Method forJournal of fungi (Basel, Switzerland) · 2022Article
- Establishment of CRISPR/Cas9 Genome-Editing System Based on Dual sgRNAs inJournal of fungi (Basel, Switzerland) · 2022Article
- A methyltransferase LaeA regulates ganoderic acid biosynthesis inFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Targeted gene insertion or replacement is a promising genome-editing tool for molecular breeding and gene engineering. Although CRISPR/Cas9 works well for gene disruption and deletion in Ganoderma lucidum, targeted gene insertion and replacement remain a serious challenge due to the low efficiency of homologous recombination (HR) in this species. In this work, we demonstrate that the DNA double-strand breaks induced by Cas9 were mainly repaired via the nonhomologous end joining (NHEJ) pathway, at a frequency of 96.7%. To establish an efficient target gene insertion and replacement tool in
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