ArticleMethods in molecular biology (Clifton, N.J.)2021
Retroviral Transduction of NKT Hybridoma Cells.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Natural killer T (NKT) cells have been shown to bridge innate and adaptive immunity. However, the rare population and hard-to-transfect of primary NKT cells slow down our understanding of cellular and molecular mechanisms of NKT development and function. To overcome these drawbacks, NKT hybridomas, especially DN32.D3 cells, are applied to study NKT cells in vitro and becoming a valuable tool. Here, we describe the method in the genetic manipulation of DN32.D3 cells by retrovirus, including the generation and concentration of retrovirus, retroviral transduction of DN32.D3 cells, and evaluation of transduction efficiency.
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