ArticleThe EMBO journal2021
MtNPF6.5 mediates chloride uptake and nitrate preference in Medicago roots.
Article in The EMBO journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- A systematic review to identify target genes that modulate root system architecture in response to abiotic stress.Scientific reports · 2025Pooled it
- The Dual-Function of CtrNAC019-CtrNPF2.1 Module in Salt Tolerance and Nitrogen Use Efficiency Via Enhancing Vacuolar Chloride Sequestration and Nitrate Efflux in Citrus trifoliata.Plant biotechnology journal · 2026Article
- Comprehensive genome-wide identification of the NPF gene family and functional characterization of GmNPF6.8 regulating root development in soybean.BMC plant biology · 2026Article
- Functional characterization of NRT1/PTR FAMILY transporters: looking for a needle in a haystack.The New phytologist · 2026Review
- Plants maximise chloride uptake during early vegetative development to stimulate cell expansion, maturation of the photosynthetic apparatus, and growth.The Plant journal : for cell and molecular biology · 2025Article
- Root-specific expression ofHorticulture research · 2025Article
- Nutrient-dependent regulation of symbiotic nitrogen fixation in legumes.Horticulture research · 2025Article
- How to Cope With Stress in the Desert-The Date Palm Approach.Plant, cell & environment · 2025Review
- Functional and Molecular Characterization of Plant Nitrate Transporters Belonging to NPF (NRT1/PTR) 6 Subfamily.International journal of molecular sciences · 2024Review
- Integrative multi-omics analyses of date palm (Phoenix dactylifera) roots and leaves reveal how the halophyte land plant copes with sea water.The plant genome · 2024Article
- Genome-wide analysis of long noncoding RNAs in response to salt stress in Nicotiana tabacum.BMC plant biology · 2023Article
- Review
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2023Article
- The small peptide CEP1 and the NIN-like protein NLP1 regulate NRT2.1 to mediate root nodule formation across nitrate concentrations.The Plant cell · 2023Article
- Genome-Wide Identification ofGenes · 2022Article
- Review
- Genome-wide identification and characterization of NPF family reveals NtNPF6.13 involving in salt stress inFrontiers in plant science · 2022Article
- Root-Specific Expression ofFrontiers in plant science · 2022Article
- MtNPF6.5 mediates chloride uptake and nitrate preference in Medicago roots.The EMBO journal · 2021Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
The preference for nitrate over chloride through regulation of transporters is a fundamental feature of plant ion homeostasis. We show that Medicago truncatula MtNPF6.5, an ortholog of Arabidopsis thaliana AtNPF6.3/NRT1.1, can mediate nitrate and chloride uptake in Xenopus oocytes but is chloride selective and that its close homologue, MtNPF6.7, can transport nitrate and chloride but is nitrate selective. The MtNPF6.5 mutant showed greatly reduced chloride content relative to wild type, and MtNPF6.5 expression was repressed by high chloride, indicating a primary role for MtNPF6.5 in root chloride uptake. MtNPF6.5 and MtNPF6.7 were repressed and induced by nitrate, respectively, and these responses required the transcription factor MtNLP1. Moreover, loss of MtNLP1 prevented the rapid switch from chloride to nitrate as the main anion in nitrate-starved plants after nitrate provision, providing insight into the underlying mechanism for nitrate preference. Sequence analysis revealed three sub-types of AtNPF6.3 orthologs based on their predicted substrate-binding residues: A (chloride selective), B (nitrate selective), and C (legume specific). The absence of B-type AtNPF6.3 homologues in early diverged plant lineages suggests that they evolved from a chloride-selective MtNPF6.5-like protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.