ArticleScientific reports2021
Effects of brief sodium fluoride treatments on the growth of early and mature cariogenic biofilms.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Effect of silver diamine fluoride and nanosilver on salivary bacterial counts in children with early childhood caries: a randomized controlled clinical trial.BMC oral health · 2025Trial
- Antimicrobial Effect of Fluoride-Generating Nonthermal Atmospheric Plasmas on In Vitro Triple-Species Oral Biofilms.Journal of functional biomaterials · 2026Article
- Comparative effectiveness of remineralization agents on attachment-associated enamel demineralization in clear aligner patients: A 6-Month DIAGNOdent-Based controlled clinical trial.BMC oral health · 2025Article
- Impact of Different Regimens of Fluoridated Dentifrice Application on the pH and Inorganic Composition in an Oral Microcosm Biofilm Model.Microorganisms · 2025Article
- In-Vitro Effect of Manuka Honey / Propolis Toothpastes on Bacteria and Biofilm Associated with Caries and Gingivitis.Oral health & preventive dentistry · 2025Article
- Comparative measurement of short-term fluoride release and inhibition of caries around restoration by ion releasing restorative materials: an in vitro study.Scientific reports · 2025Article
- Effects of Tooth Desensitizers onInternational journal of molecular sciences · 2024Article
- Comprehensive in vitro and in ovo assessment of cytotoxicity: Unraveling the impact of sodium fluoride, xylitol, and their synergistic associations in dental products.Biomolecules & biomedicine · 2024Article
- Fluoride-Induced Mitochondrial Dysfunction and Approaches for Its Intervention.Biological trace element research · 2024Review
- Measurement and analysis of microbial fluoride resistance in dental biofilm models.Methods in enzymology · 2024Article
- Hydroxyapatite Pellets as Versatile Model Surfaces for Systematic Adhesion Studies on Enamel: A Force Spectroscopy Case Study.ACS biomaterials science & engineering · 2022Article
- Effect of Fluoride on Cytotoxicity Involved in Mitochondrial Dysfunction: A Review of Mechanism.Frontiers in veterinary science · 2022Review
- Effect of gastric acid on the surface roughness and bacterial adhesion of bulk-fill composite resins.Brazilian dental journalArticle
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although fluoride has been widely used as a preventive agent for dental caries, the effects of fluoride on the activities of biofilms in different stages of cariogenic biofilm formation are less studied. This study was designed to investigate the antibiofilm activity of sodium fluoride during the early and mature stages of Streptococcus mutans (S. mutans) biofilm formation. S. mutans biofilms were formed on saliva-coated hydroxyapatite disks. In the early (0-46 h) and mature (46-94 h) biofilm stages, the biofilms were treated with different concentrations of fluoride (250, 500, 1000, 2000 ppm; 5 times in total, 1 min/treatment). Acidogenicity, dry weight, colony-forming units (CFUs), water-soluble/insoluble extracellular polysaccharides (EPSs), and intracellular polysaccharides were analysed, and confocal laser scanning microscopy images were obtained of the two stages of biofilms to determine antibiofilm activities of fluoride at varying concentrations during the formation of early and mature biofilms. In the early stages of cariogenic biofilm formation, test groups with all fluoride concentrations significantly inhibited the growth of S. mutans biofilms. The antibiofilm and anti-EPS formation activities of the brief fluoride treatments increased with a concentration-dependent pattern. At the mature biofilm stage, only the 2000 ppm fluoride treatment group significantly inhibited biofilm accumulation, activity, and intracellular/extracellular polysaccharide content compared with those of the control and other fluoride treatment groups. The antimicrobial effect of fluoride treatment on the growth of S. mutans biofilms was linked with the stage of cariogenic biofilm formation. The inhibition of S. mutans biofilm growth by fluoride treatment was easier in the early formation stage than in the mature stage. Fluoride treatment in the early stage of cariogenic biofilm formation may be an effective approach to controlling cariogenic biofilm development and preventing dental caries.
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