ArticleVeterinary pathology2022
Flow cytometric analysis of equine bronchoalveolar lavage fluid cells in horses with and without severe equine asthma.
Article in Veterinary pathology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Immunohistological characterization of equine synovial tissue in metacarpophalangeal joints of different ages and osteoarthritis status.Osteoarthritis and cartilage open · 2026Article
- Exploring a pico-well based scRNA-seq method (HIVE) for simplified processing of equine bronchoalveolar lavage cells.PloS one · 2025Article
- Detection of fungi in the airways of horses according to the sample site: a methodological study.Veterinary research communications · 2024Article
- Effect of age, season, and gender on bronchoalveolar lavage fluid cytology in camels.Open veterinary journal · 2023Article
- Equine alveolar macrophages and monocyte-derived macrophages respond differently to an inflammatory stimulus.PloS one · 2023Article
- Highly efficacious and safe neutralizing DNA aptamer of SARS-CoV-2 as an emerging therapy for COVID-19 disease.Virology journal · 2022Article
- Giant Multinucleated Cells Are Associated with Mastocytic Inflammatory Signature Equine Asthma.Animals : an open access journal from MDPI · 2022Article
- The Immune Mechanisms of Severe Equine Asthma-Current Understanding and What Is Missing.Animals : an open access journal from MDPI · 2022Review
- Initial investigation of molecular phenotypes of airway mast cells and cytokine profiles in equine asthma.Frontiers in veterinary science · 2022Article
- Single-cell gene expression analysis of cryopreserved equine bronchoalveolar cells.Frontiers in immunology · 2022Article
- Comprehensive Flow Cytometric Characterization of Bronchoalveolar Lavage Cells Indicates Comparable Phenotypes Between Asthmatic and Healthy Horses But Functional Lymphocyte Differences.Frontiers in immunology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Severe equine asthma (SEA) is a common, debilitating lower airway inflammatory disorder of older horses. Alveolar macrophages (AMs) survey inhaled particulates from barn sources causing them to switch from an anti-inflammatory to a proinflammatory phenotype, resulting in neutrophil recruitment to the lung. This proinflammatory switch may contribute to the development and prolongation of SEA. Validated antibodies to identify the cells involved in the pathogenesis of SEA are lacking. In this study, monoclonal antibodies against CD90, CD163, and CD206 were tested for reactivity with equine leukocytes by immunocytochemistry and flow cytometry. A multi-color flow cytometric assay was developed to identify leukocytes in equine bronchoalveolar lavage fluid (BALF). Four control and 4 SEA-susceptible horses had BALF collected before and after a 48-hour moldy hay challenge. Antibodies against CD90 uniquely labeled equine neutrophils, and antibodies against CD163 and CD206 identified equine macrophages. Postchallenge AM surface expression of CD163 increased in both groups of horses, but the increase was statistically significant in only the SEA-susceptible group (
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