Evidence map›Paper›PMID 34520015›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Biofabrication of 3D Human Muscle Model with Vascularization and Endomysium.

Simone Bersini, Riccardo Francescato, Matteo Moretti

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Simone BersiniRegenerative Medicine Technologies Laboratory, Ente Ospedaliero Cantonale, Lugano, Switzerland.
Riccardo FrancescatoRegenerative Medicine Technologies Laboratory, Ente Ospedaliero Cantonale, Lugano, Switzerland.
Matteo MorettiRegenerative Medicine Technologies Laboratory, Ente Ospedaliero Cantonale, Lugano, Switzerland. matteo.moretti@eoc.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This protocol describes the biofabrication of 3D millimeter-scale human muscle units, embedding non-planar muscle fibers wrapped by fibroblasts-rich endomysium and intertwined with microvascular networks. Suspended muscle fibers are formed through the self-assembly of human myoblasts within cylindrical cavities generated in a sacrificial gelatin template cast in a 3D printed frame. Following myotube differentiation, muscle fibers are embedded in a 3D matrix containing endothelial cells and muscle-derived fibroblasts. The cellular complexity of the environment is instrumental to drive fibroblast migration towards muscle fibers and to induce the organ-specific differentiation of endothelial cells. This advanced 3D muscle model can be applied to analyze the biological mechanisms underlying specific muscle diseases which involve a complex remodeling of the muscle environment (e.g., muscular dystrophies and fibrosis) whereby the pathological interplay among different cell populations drives the onset and progression of the disease.

Indexed as

Endothelial CellsMyocardiumHumansMuscle Fibers, SkeletalMuscular Dystrophy, DuchenneMyoblasts3D human muscle model3D printingDuchenne muscular dystrophyEndomysiumEndothelial cellFibrosisMesoscaleOrgan specificityVasculature

Identifiers

PMID34520015

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.