ArticleNicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco2022
Chronic E-Cigarette Exposure Alters Human Alveolar Macrophage Morphology and Gene Expression.
Article in Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- The triple-hit hypothesis: exploring pulmonary e-cigarette, PMEuropean respiratory review : an official journal of the European Respiratory Society · 2026Review
- Impact of e-cigarette vaping on the immune system across the body.Physiological reviews · 2026Review
- Article
- Polarization of THP-1-Derived Human M0 to M1 Macrophages Exposed to Flavored E-Liquids.Toxics · 2025Article
- The effects of flavored vaped e-liquids on cultured human macrophages derived from the central and peripheral nervous systems.Toxicology in vitro : an international journal published in association with BIBRA · 2025Article
- Immunological Effects of Electronic Cigarette Use: A Review of Current Evidence.Clinical reviews in allergy & immunology · 2025Review
- E-cigarette vapor amplifies neutrophilic inflammation and proteolytic EVs in response to LPS.Frontiers in immunology · 2025Article
- Severe Pulmonary Blastomycosis in a Young Adult: Probable Role of E-Cigarette Use in Immunosuppression.Case reports in medicine · 2025Article
- Evaluation of E-Cigarette Effects on Gene Expression Profile of Human Bronchial Epithelial Cells: A System Biology Approach.Tanaffos · 2025Article
- Airway Proteotypes of E-Cigarette Users Overlap with Those Found in Asthmatics.American journal of respiratory cell and molecular biology · 2024Article
- Sex-specific lung inflammation and mitochondrial damage in a model of electronic cigarette exposure in asthma.American journal of physiology. Lung cellular and molecular physiology · 2023Article
- The lipolysis inhibitor acipimox reverses the cardiac phenotype induced by electronic cigarettes.Scientific reports · 2023Article
- Molecular Imaging of Pulmonary Inflammation in Users of Electronic and Combustible Cigarettes: A Pilot Study.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2023Article
- Pro-inflammatory effects of aerosols from e-cigarette-derived flavoring chemicals on murine macrophages.Toxicology reports · 2023Article
- ERS International Congress 2021: highlights from the Epidemiology and Environment Assembly.ERJ open research · 2022Article
- Inflammatory phenotype modulation in the respiratory tract and systemic circulation of e-cigarette users: a pilot study.American journal of physiology. Lung cellular and molecular physiology · 2021Article
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Authors and funding
12 authors.
Funding
Abstract
introductionAlveolar macrophages (AMs) are lung-resident immune cells that phagocytose inhaled particles and pathogens, and help coordinate the lung's immune response to infection. Little is known about the impact of chronic e-cigarette use (ie, vaping) on this important pulmonary cell type. Thus, we determined the effect of vaping on AM phenotype and gene expression. AIMS AND
methodsWe recruited never-smokers, smokers, and e-cigarette users (vapers) and performed research bronchoscopies to isolate AMs from bronchoalveolar lavage fluid samples and epithelial cells from bronchial brushings. We then performed morphological analyses and used the Nanostring platform to look for changes in gene expression.
resultsAMs obtained from smokers and vapers were phenotypically distinct from those obtained from nonsmokers, and from each other. Immunocytochemistry revealed that vapers AMs had significantly elevated inducible nitric oxide synthase (M1) expression and significantly reduced CD301a (M2) expression compared with nonsmokers or smokers. Vapers' AMs and bronchial epithelia exhibited unique changes in gene expression compared with nonsmokers or smokers. Moreover, vapers' AMs were the most affected of all groups and had 124 genes uniquely downregulated. Gene ontology analysis revealed that vapers and smokers had opposing changes in biological processes.
conclusionsThese data indicate that vaping causes unique changes to AMs and bronchial epithelia compared with nonsmokers and smokers which may impact pulmonary host defense. IMPLICATIONS: These data indicate that normal "healthy" vapers have altered AMs and may be at risk of developing abnormal immune responses to inflammatory stimuli.
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