ArticlemSystems2021
Species-Level Resolution of Female Bladder Microbiota from 16S rRNA Amplicon Sequencing.
Article in mSystems, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- A rapid ionic liquid-based DNA extraction method for molecular diagnostics of urinary tract infections.Microbiology spectrum · 2026Article
- Multi-Kingdom Fecal Microbiota Alterations in Horses with Severe Equine Asthma.Microorganisms · 2026Article
- Microbial next generation DNA sequencing of aspirated synovial fluid shows concordance with ICM criteria biomarkers for diagnosing periprosthetic joint infection in hip and knee arthroplasty.Frontiers in microbiology · 2026Article
- Research advances on gut microbiota dysbiosis and chronic liver diseases: a review.Frontiers in medicine · 2026Review
- The Urinary Microbiota and the Gut-Bladder Axis in Bladder Cancer.International journal of molecular sciences · 2025Review
- Beyond Infection: How Antimicrobial Therapies Influence the Urinary Microbiome and Stone Disease.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Clinical Evaluation of Microbial Communities and Associated Biofilms with Breast Augmentation Failure.Microorganisms · 2024Article
- GutJHEP reports : innovation in hepatology · 2024Article
- Synthesis of current pediatric urinary microbiome research.Frontiers in pediatrics · 2024Review
- Effects of Fishmeal Substitution with Mealworm Meals (Aquaculture nutrition · 2024Article
- Comparative Effectiveness Randomized Clinical Trial Using Next-generation Microbial Sequencing to Direct Prophylactic Antibiotic Choice Before Urologic Stone Lithotripsy Using an Interprofessional Model.European urology open science · 2023Article
- The newest Oxford Nanopore R10.4.1 full-length 16S rRNA sequencing enables the accurate resolution of species-level microbial community profiling.Applied and environmental microbiology · 2023Article
- Application of Various Techniques to Gain Insights Into the Complex Urinary Tract Microbial Communities of Renal Transplant Recipients.Transplantation direct · 2023Article
- Supplementation ofMicroorganisms · 2023Article
- Cataloging variation in 16S rRNA gene sequences of female urobiome bacteria.Frontiers in urology · 2023Article
- Urinary Microbiome of Reproductive-Age Asymptomatic European Women.Microbiology spectrum · 2022Article
- Microbiome in Male Genital Mucosa (Prepuce, Glans, and Coronal Sulcus): A Systematic Review.Microorganisms · 2022Article
- Article
- Combination of Whole Genome Sequencing and Metagenomics for Microbiological Diagnostics.International journal of molecular sciences · 2022Review
- Choice of 16S Ribosomal RNA Primers Impacts Male Urinary Microbiota Profiling.Frontiers in cellular and infection microbiology · 2022Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The human bladder contains bacteria, even in the absence of infection. Interest in studying these bacteria and their association with bladder conditions is increasing. However, the chosen experimental method can limit the resolution of the taxonomy that can be assigned to the bacteria found in the bladder. 16S rRNA amplicon sequencing is commonly used to identify bacteria in urinary specimens, but it is typically restricted to genus-level identification. Our primary aim here was to determine if accurate species-level identification of bladder bacteria is possible using 16S rRNA amplicon sequencing. We evaluated the ability of different classification schemes, each consisting of combinations of a reference database, a 16S rRNA gene variable region, and a taxonomic classification algorithm to correctly classify bladder bacteria. We show that species-level identification is possible and that the reference database chosen is the most important component, followed by the 16S variable region sequenced.
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Registered trials
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