Evidence map›Paper›PMID 34519222›Full record

ArticleBioTechniques2021

Improved SARS-CoV-2 PCR detection and genotyping with double-bubble primers.

Menachem Ailenberg, Andras Kapus, Ori D Rotstein

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioTechniques, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Menachem AilenbergKeenan Research Centre for Biomedical Science of St Michael's Hospital, Unity Health Toronto & The Departments of Surgery, St Michael's Hospital & The University of Toronto, Toronto, Canada.
Andras KapusKeenan Research Centre for Biomedical Science of St Michael's Hospital, Unity Health Toronto & The Departments of Surgery, St Michael's Hospital & The University of Toronto, Toronto, Canada.
Ori D RotsteinKeenan Research Centre for Biomedical Science of St Michael's Hospital, Unity Health Toronto & The Departments of Surgery, St Michael's Hospital & The University of Toronto, Toronto, Canada.
St. Michael's Hospital · CAUniversity of Toronto · CA

Funding

CIHR 400642St. Michael's Hospital Foundation
6 · The paper itself

Abstract

A new approach for improved RT-PCR is described. It is based on primers designed to form controlled stem-loop and homodimer configurations, hence the name 'double-bubble' primers. The primers contain three main regions for efficient RT-PCR: a 3' short overhang to allow reverse transcription, a stem region for hot start and a template-specific region for PCR amplification. As proof of principle,

Indexed as

COVID-19COVID-19 Nucleic Acid TestingPolymerase Chain ReactionSARS-CoV-2DNA PrimersGenotypeHumansRNA, ViralSensitivity and SpecificityDNA PrimersRNA, Viralcost-effective PCRdouble-bubble PCR primersfast PCRhot-start PCRRT-qPCRSARS-CoV-2

Identifiers

PMID34519222
PMCPMC8544493
OpenAlexW3199669378

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.