ArticleBiosafety and health2021
The impact of sample processing on the rapid antigen detection test for SARS-CoV-2: Virus inactivation, VTM selection, and sample preservation.
Article in Biosafety and health, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Emerging strategies for monkeypox: antigen and antibody applications in diagnostics, vaccines, and treatments.Military Medical Research · 2025Review
- The establishment of a universal standard for both viral antigen and nucleid acid detection based on digital PCR.Virology journal · 2025Article
- Rolosense: Mechanical Detection of SARS-CoV-2 Using a DNA-Based Motor.ACS central science · 2024Article
- Ammonium sulfate denatures transport medium less dependent on guanidinium isothiocyanate and enables SARS-CoV-2 RNA and antigen detection compatibility.Frontiers in microbiology · 2024Article
- Sandwich ELISA for the Quantification of Nucleocapsid Protein of SARS-CoV-2 Based on Polyclonal Antibodies from Two Different Species.International journal of molecular sciences · 2023Article
- Establishment and application of national reference panels for SARS-CoV-2 antigen detection kit.Biosafety and health · 2023Article
- Results of German external quality assessment schemes for SARS-CoV-2 antigen detection.Scientific reports · 2023Article
- How to better select SARS-CoV-2 preservation solution of virus nucleic acid testing.Journal of clinical laboratory analysis · 2023Article
- Article
- Diagnostic performance of a novel antigen-capture ELISA for the detection of SARS-CoV-2.Analytical biochemistry · 2023Article
- Article
- Performance Evaluation of Five Rapid At-Home COVID-19 Antigen Tests against the Omicron Variant.Microbiology spectrum · 2023Article
- Evaluation of diagnostic performance of SARS-CoV-2 detection kits: a comparative study.Beni-Suef University journal of basic and applied sciences · 2023Article
- China's NMPA perspective on the clinical performance of SARS-CoV-2 antigen test reagents.Bioanalysis · 2022Review
- A Potent Neutralizing Nanobody Targeting the Spike Receptor-Binding Domain of SARS-CoV-2 and the Structural Basis of Its Intimate Binding.Frontiers in immunology · 2022Article
- Development of a rapid, simple, and sensitive point-of-care technology platform utilizing ternary NanoLuc.Frontiers in microbiology · 2022Article
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Authors and funding
8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Many factors have been identified as having the ability to affect the sensitivity of rapid antigen detection (RAD) tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This study aimed to identify the impact of sample processing on the sensitivity of the RAD tests. We explored the effect of different inactivation methods, viral transport media (VTM) solutions, and sample preservation on the sensitivity of four RAD kits based on two SARS-CoV-2 strains. Compared with non-inactivation, heat inactivation significantly impacted the sensitivity of most RAD kits; however, β-propiolactone inactivation only had a minor effect. Some of the VTM solutions (VTM2, MANTACC) had a significant influence on the sensitivity of the RAD kits, especially for low viral-loads samples. The detection value of RAD kits was slightly decreased, while most of them were still in the detection range with the extension of preservation time and the increase of freeze-thaw cycles. Our results showed that selecting the appropriate inactivation methods and VTM solutions is necessary during reagent development, performance evaluation, and clinical application.
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