ArticleNPJ Regenerative medicine2021
The Warburg effect is necessary to promote glycosylation in the blastema during zebrafish tail regeneration.
Article in NPJ Regenerative medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 54 citations in OpenAlex.
- TGFβ-dependent upregulation of OCIAD2 is essential for epithelial-to-mesenchymal transition during mesendoderm differentiation.Stem cell reports · 2026Article
- The microenvironment dictates glyco-immune surveillance via HSF1-mediated metabolism.Science advances · 2026Article
- The glycobiology of prostate cancer: an update.Oncogene · 2026Review
- Hcfc1 and Ogt Mediate Zebrafish CNS Regeneration Through Hippo/Yap Signalling.Cell proliferation · 2026Article
- Signal control during tissue regeneration in adult animals.Nature reviews. Molecular cell biology · 2026Review
- In vivo screen reveals specific roles of Hippo pathway components in development and regeneration.Life science alliance · 2026Article
- Metabolic reprogramming regulates histone lactylation during zebrafish caudal fin regeneration.iScience · 2026Article
- The evolutionary origin and mechanism of chordate tail regeneration. An ancient tale?Cells & development · 2025Review
- TGF-beta coordinates changes in Keratin gene expression during complex tissue regeneration.Scientific reports · 2025Article
- Active mitochondria in healthy spiny mouse fibroblasts resemble megamitochondria and remain resilient across lifespan.bioRxiv : the preprint server for biology · 2025Article
- Mammalian Blastema: Possibility and Potentials.International journal of stem cells · 2025Review
- Article
- Epithelial-mesenchymal transition in tissue repair and degeneration.Nature reviews. Molecular cell biology · 2024Review
- Imaging immunometabolismImmunometabolism (Cobham, Surrey) · 2024Article
- Identification of distinct vascular mural cell populations during zebrafish embryonic development.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- Developmental regulation of cellular metabolism is required for intestinal elongation and rotation.Development (Cambridge, England) · 2024Article
- Adaptation of Glucose Metabolism to Limb Autotomy and Regeneration in the Chinese Mitten Crab.Marine biotechnology (New York, N.Y.) · 2024Article
- Anti-inflammatory effects and beneficial effects of the feed additive Urtica cannabina L. in zebrafish.PloS one · 2024Article
- Molecular basis of retinal remodeling in a zebrafish model of retinitis pigmentosa.Cellular and molecular life sciences : CMLS · 2023Article
- Enduring questions in regenerative biology and the search for answers.Communications biology · 2023Review
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
Throughout their lifetime, fish maintain a high capacity for regenerating complex tissues after injury. We utilized a larval tail regeneration assay in the zebrafish Danio rerio, which serves as an ideal model of appendage regeneration due to its easy manipulation, relatively simple mixture of cell types, and superior imaging properties. Regeneration of the embryonic zebrafish tail requires development of a blastema, a mass of dedifferentiated cells capable of replacing lost tissue, a crucial step in all known examples of appendage regeneration. Using this model, we show that tail amputation triggers an obligate metabolic shift to promote glucose metabolism during early regeneration similar to the Warburg effect observed in tumor forming cells. Inhibition of glucose metabolism did not affect the overall health of the embryo but completely blocked the tail from regenerating after amputation due to the failure to form a functional blastema. We performed a time series of single-cell RNA sequencing on regenerating tails with and without inhibition of glucose metabolism. We demonstrated that metabolic reprogramming is required for sustained TGF-β signaling and blocking glucose metabolism largely mimicked inhibition of TGF-β receptors, both resulting in an aberrant blastema. Finally, we showed using genetic ablation of three possible metabolic pathways for glucose, that metabolic reprogramming is required to provide glucose specifically to the hexosamine biosynthetic pathway while neither glycolysis nor the pentose phosphate pathway were necessary for regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.