ArticleBMC plant biology2021
Development of a Csy4-processed guide RNA delivery system with soybean-infecting virus ALSV for genome editing.
Article in BMC plant biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Advances in CRISPR multiplex gene editing to map and modify stress-responsive transcription factor networks for crop improvement.Plant cell reports · 2026Review
- Turnip Mosaic Virus-Based gRNA Delivery System for Plant Genome Editing.Plant direct · 2026Article
- A portable Cas6f-based system for multiplex translational repression in bacteria.Nature communications · 2026Article
- Advances in Tissue Culture-Free Genetic Engineering and Genome Editing of Peanut.Molecular biotechnology · 2026Review
- CRISPR/Cas-Mediated Optimization of Soybean Shoot Architecture for Enhanced Yield.International journal of molecular sciences · 2025Review
- CRISPR/Cas genome editing in soybean: challenges and new insights to overcome existing bottlenecks.Journal of advanced research · 2025Review
- Non-tissue culture genetic modifications for plant improvement.Plant molecular biology · 2025Review
- Virus-Induced Genome Editing (VIGE): One Step Away from an Agricultural Revolution.International journal of molecular sciences · 2025Review
- Efficient mutagenesis and genotyping of maize inbreds using biolistics, multiplex CRISPR/Cas9 editing, and Indel-Selective PCR.Plant methods · 2025Article
- Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing.Plants (Basel, Switzerland) · 2025Review
- Trans-complementation of the viral movement protein mediates efficient expression of large target genes via a tobacco mosaic virus vector.Plant biotechnology journal · 2024Article
- Soybean-Phakopsora pachyrhizi interactions: towards the development of next-generation disease-resistant plants.Plant biotechnology journal · 2024Review
- Foxtail mosaic virus: A tool for gene function analysis in maize and other monocots.Molecular plant pathology · 2023Review
- Establishing a Virus-Induced Gene Silencing System inPlants (Basel, Switzerland) · 2023Article
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
- Recalcitrance to transformation, a hindrance for genome editing of legumes.Frontiers in genome editing · 2023Review
- Virus-Induced Gene Editing and Its Applications in Plants.International journal of molecular sciences · 2022Review
- CRISPR/Cas9 in Planta Hairy Root Transformation: A Powerful Platform for Functional Analysis of Root Traits in Soybean.Plants (Basel, Switzerland) · 2022Review
- Advances in gene editing without residual transgenes in plants.Plant physiology · 2022Article
- Progress in Soybean Genetic Transformation Over the Last Decade.Frontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA key issue for implementation of CRISPR-Cas9 genome editing for plant trait improvement and gene function analysis is to efficiently deliver the components, including guide RNAs (gRNAs) and Cas9, into plants. Plant virus-based gRNA delivery strategy has proven to be an important tool for genome editing. However, its application in soybean which is an important crop has not been reported yet. ALSV (apple latent spherical virus) is highly infectious virus and could be explored for delivering elements for genome editing.
resultsTo develop a ALSV-based gRNA delivery system, the Cas9-based Csy4-processed ALSV Carry (CCAC) system was developed. In this system, we engineered the soybean-infecting ALSV to carry and deliver gRNA(s). The endoribonuclease Csy4 effectively releases gRNAs that function efficiently in Cas9-mediated genome editing. Genome editing of endogenous phytoene desaturase (PDS) loci and exogenous 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) sequence in Nicotiana. benthamiana (N. benthamiana) through CCAC was confirmed using Sanger sequencing. Furthermore, CCAC-induced mutagenesis in two soybean endogenous GW2 paralogs was detected.
conclusionsWith the aid of the CCAC system, the target-specific gRNA(s) can be easily manipulated and efficiently delivered into soybean plant cells by viral infection. This is the first virus-based gRNA delivery system for soybean for genome editing and can be used for gene function study and trait improvement.
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