ArticleJournal of the American Chemical Society2021
Design of Large Stokes Shift Fluorescent Proteins Based on Excited State Proton Transfer of an Engineered Photobase.
Article in Journal of the American Chemical Society, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 83 citations in OpenAlex.
- Unraveling Solvent-Independent Excited State Proton Transfer Dynamics in Sterically Substituted Photoactive Systems.The journal of physical chemistry letters · 2026Article
- De Novo Design of Near-Infrared Fluorescence-Activating Proteins.Journal of the American Chemical Society · 2026Article
- Photoisomerization detected in a fully wavelength-tunable rhodopsin mimic system.Acta crystallographica. Section D, Structural biology · 2026Article
- Long-Stokes-Shift mScarlet3 as a Structural Marker for Two-Photon Imaging.microPublication biology · 2026Article
- Synthesis of Novel Phenanthroimidazole Based Beta-Diketone Compounds: Investigation of Their Spectroscopic Properties and Electrochemical Characterization.Journal of fluorescence · 2025Article
- Selenium-Substituted BOIMPY for Enhanced Photodynamic Therapy.ACS medicinal chemistry letters · 2025Article
- Dimerizing Heptamethine Cyanine Fluorophores from the Meso Position: Synthesis, Optical Properties, and Metal Sensing Studies.Organic letters · 2025Article
- Near-infrared II cyanine fluorophores with large stokes shift engineered by regulating respective absorption and emission.Nature communications · 2025Article
- pH-Induced Conformational Change of the Chromophore of the Large Stokes Shift Fluorescent Protein tKeima.Molecules (Basel, Switzerland) · 2025Article
- Aggregation-Induced Luminescent 3-Phenylpyrano[4,3-b]quinolizine Derivatives as Photosensitizers with Anti-Cancer Properties.Molecules (Basel, Switzerland) · 2025Article
- pH-responsive supramolecular switch of a rationally designed dipyrroethene-based chromophore.Chemical science · 2025Article
- Structural basis of a small monomeric Clivia fluorogenic RNA with a large Stokes shift.Nature chemical biology · 2024Article
- Regulation of Absorption and Emission in a Protein/Fluorophore Complex.ACS chemical biology · 2024Article
- Excited State Proton Transfer from Acidic Alcohols to a Quinoline Photobase Can Be Solvated by Non-Acidic Alcohol Solvents.The journal of physical chemistry. A · 2024Article
- Structural Analysis of the Large Stokes Shift Red Fluorescent Protein tKeima.Molecules (Basel, Switzerland) · 2024Article
- PyrAtes: Modular Organic Salts with Large Stokes Shifts for Fluo-rescence Microscopy.Angewandte Chemie (International ed. in English) · 2024Article
- Unprecedented perspectives on the application of CinNapht fluorophores provided by a "late-stage" functionalization strategy.Chemical science · 2023Article
- Introduction of Session 1, "Photochemistry of retinal proteins".Biophysics and physicobiology · 2023Article
- Light controlled reversible Michael addition of cysteine: a new tool for dynamic site-specific labeling of proteins.The Analyst · 2023Article
- Synthesis of Fluorescent, Dumbbell-Shaped Polyurethane Homo- and Heterodendrimers and Their Photophysical Properties.International journal of molecular sciences · 2023Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
The incredible potential for fluorescent proteins to revolutionize biology has inspired the development of a variety of design strategies to address an equally broad range of photophysical characteristics, depending on potential applications. Of these, fluorescent proteins that simultaneously exhibit high quantum yield, red-shifted emission, and wide separation between excitation and emission wavelengths (Large Stokes Shift, LSS) are rare. The pursuit of LSS systems has led to the formation of a complex, obtained from the marriage of a rationally engineered protein (human cellular retinol binding protein II, hCRBPII) and different fluorogenic molecules, capable of supporting photobase activity. The large increase in basicity upon photoexcitation leads to protonation of the fluorophore in the excited state, dramatically red-shifting its emission, leading to an LSS protein/fluorophore complex. Essential for selective photobase activity is the intimate involvement of the target protein structure and sequence that enables Excited State Proton Transfer (ESPT). The potential power and usefulness of the strategy was demonstrated in live cell imaging of human cell lines.
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