ArticleArtificial organs2022
Neutrophil dysfunction due to continuous mechanical shear exposure in mechanically assisted circulation in vitro.
Article in Artificial organs, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Circulating MIF, D-DT, and Soluble CD74 in End-Stage Heart Failure Patients Receiving LVAD: An Exploratory Clinical Study and Effects on Adult Cardiac Myofibroblasts.Biomedicines · 2026Article
- A miniaturized mouse extracorporeal circulation model to characterize early hematologic and metabolic alterations.Frontiers in physiology · 2026Article
- In Vitro Comparison of Device-Induced Hemolysis, Platelet Defects, and von Willebrand Factor Degradation Between the HeartMate 2 and HeartMate 3 Pumps.Artificial organs · 2025Article
- A Biophysics-Based Mathematical Model of Shear-Induced Platelet Activation and Receptor Shedding: Re-Examining Previous Experimental Data.ASAIO journal (American Society for Artificial Internal Organs : 1992) · 2025Article
- In vitro study on device-induced damage to blood cellular components and degradation of von Willebrand factor in a CentriMag pump-assisted circulation.Artificial organs · 2024Article
- Increased phagocytosis capacity of circulating neutrophils in patients on continuous flow ventricular assist device support.Artificial organs · 2024Article
- The Disconnect Between Extracorporeal Circulation and the Microcirculation: A Review.ASAIO journal (American Society for Artificial Internal Organs : 1992) · 2022Review
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Authors and funding
7 authors.
Funding
Abstract
objectiveLeukocytes play an important role in the body's immune system. The aim of this study was to assess alterations in neutrophil phenotype and function in pump-assisted circulation in vitro.
methodsHuman blood was circulated for four hours in three circulatory flow loops with a CentriMag blood pump operated at a flow of 4.5 L/min at three rotational speeds (2100, 2800, and 4000 rpm), against three pressure heads (75, 150, and 350 mm Hg), respectively. Blood samples were collected hourly for analyses of neutrophil activation state (Mac-1, CD62L, CD162), neutrophil reactive oxygen species (ROS) production, apoptosis, and neutrophil phagocytosis.
resultsActivated neutrophils indicated by both Mac-1 expression and decreased surface expression of CD62L and CD162 receptors increased with time in three loops. The highest level of neutrophil activation was observed in the loop with the highest rotational speed. Platelet-neutrophil aggregates (PNAs) progressively increased in two loops with lower rotational speeds. PNAs peaked at one hour after circulation and decreased subsequently in the loop with the highest rotational speed. Neutrophil ROS production dramatically increased at one hour after circulation and decreased subsequently in all three loops with similar levels and trends. Apoptotic neutrophils increased with time in all three loops. Neutrophil phagocytosis capacity in three loops initially elevated at one hour after circulation and decreased subsequently. Apoptosis and altered phagocytosis were dependent on rotational speed.
conclusionsOur study revealed that the pump-assisted circulation induced neutrophil activation, apoptosis, and functional impairment. The alterations were strongly associated with pump operating condition and duration.
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