Evidence map›Paper›PMID 34515601›Full record

ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2022

Validation of a commercial human ELISA to measure hyaluronic acid concentration in feline plasma.

Kaela E Shaw, Alexa M Bersenas, Shane W Bateman, Shauna L Blois, R Darren Wood

Open access · bronzeAbstract read
In one paragraph

Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 85% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Kaela E ShawDepartments of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.ORCID https://orcid.org/0000-0001-6640-4604
Alexa M BersenasDepartments of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Shane W BatemanDepartments of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.ORCID https://orcid.org/0000-0002-0468-0256
Shauna L BloisDepartments of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
R Darren WoodPathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
University of Guelph · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Our goal was to validate a human hyaluronic acid (HA) ELISA (Hyaluronic acid plus ELISA; TECOmedical Group) for use in feline plasma. Plasma from 5 healthy cats and 5 critically ill cats was used for validation of the assay. Validation methods performed included intra- and inter-assay variability, spike-and-recovery, and dilutional linearity. All measurements were performed in duplicate. The precision study revealed good intra-assay CV of 7.4-8.9%; inter-assay CV was 3.4-4.2%. Extraction efficiency via spiking tests yielded mean recovery of 89.6%. The assay met criteria for acceptable linearity using 3 serial dilutions. Our results demonstrate that this commercial HA ELISA had acceptable analytical performance using feline plasma and could be a useful tool in the veterinary clinical research setting.

Indexed as

Hyaluronic AcidAnimalsCatsEnzyme-Linked Immunosorbent AssayHumansHyaluronic AcidELISAendothelial glycocalyxfelinehyaluronic acidvalidation

Identifiers

PMID34515601
PMCPMC8689016
OpenAlexW3200472507

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.