ArticleJournal of cell science2021
ExBoX - a simple Boolean exclusion strategy to drive expression in neurons.
Article in Journal of cell science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 9 citations in OpenAlex.
- Localization and Functional Characterization of MDGA1 in Mouse Hippocampus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Cell-free recombinase-integrated Boolean output system.Cell systems · 2025Article
- Capicua Refines Mossy Fiber-CA3 Axon Targeting in the Late Postnatal Hippocampus.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Anterior cingulate cortex parvalbumin and somatostatin interneurons shape social behavior in male mice.Nature communications · 2025Article
- Stepwise molecular specification of excitatory synapse diversity onto cerebellar Purkinje cells.Nature neuroscience · 2025Article
- An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold.PLoS biology · 2023Article
- Optical opening of the blood-brain barrier for targeted and ultra-sparse viral infection of cells in mouse cortex.Cell reports methods · 2023Article
- A genetic tool for the longitudinal study of a subset of post-inflammatory reactive astrocytes.Cell reports methods · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The advent of modern single-cell biology has revealed the striking molecular diversity of cell populations once thought to be more homogeneous. This newly appreciated complexity has made intersectional genetic approaches essential to understanding and probing cellular heterogeneity at the functional level. Here, we build on previous knowledge to develop a simple adeno-associated virus (AAV)-based approach to define specific subpopulations of cells by Boolean exclusion logic (AND NOT). This expression by Boolean exclusion (ExBoX) system encodes for a gene of interest that is turned on by a particular recombinase (Cre or FlpO) and turned off by another. ExBoX allows for the specific transcription of a gene of interest in cells expressing only the activating recombinase, but not in cells expressing both. We show the ability of the ExBoX system to tightly regulate expression of fluorescent reporters in vitro and in vivo, and further demonstrate the adaptability of the system by achieving expression of a variety of virally delivered coding sequences in the mouse brain. This simple strategy will expand the molecular toolkit available for cell- and time-specific gene expression in a variety of systems.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.