ArticleHeliyon2021
Detection of SARS-CoV-2 spike protein D614G mutation by qPCR-HRM analysis.
Article in Heliyon, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- Application of a high-resolution melt assay for monitoring SARS-CoV-2 variants in Burkina Faso and Kenya.mSphere · 2025Article
- Rapid detection of the SARS-CoV-2 omicron variants based on high-resolution melting curve analysis.Scientific reports · 2024Article
- Detection of SARS-CoV-2 spike protein D614G mutation using μTGGE.Molecular biology reports · 2024Article
- Rapid monitoring of SARS-CoV-2 variants of concern through high-resolution melt analysis.Scientific reports · 2023Article
- Article
- High-resolution melting analysis to discriminate between the SARS-CoV-2 Omicron variants BA.1 and BA.2.Biochemistry and biophysics reports · 2022Article
- Real-time allelic assays of SARS-CoV-2 variants to enhance sewage surveillance.Water research · 2022Article
- High-resolution melting analysis after nested PCR for the detection of SARS-CoV-2 spike protein G339D and D796Y variations.Biochemical and biophysical research communications · 2022Article
- Photonics enabled intelligence system to identify SARS-CoV 2 mutations.Applied microbiology and biotechnology · 2022Review
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesMonitoring the spread of the G614 in specific locations is critical as this variant is highly transmissible and can trigger the emergence of other mutations. Therefore, a rapid and accurate method that can reliably detect the D614G mutation will be beneficial. This study aims to analyze the potential use of the two-step Reverse Transcriptase
methodsSix SARS-CoV-2 RNA samples were synthesized into cDNA and analyzed with the qPCR-HRM method in order to detect the D614G mutation in Spike protein of SARS-CoV-2. The primers are designed to target the specific Spike region containing the D614G mutation. The qPCR-HRM analysis was conducted simultaneously, and the identification of the SARS-CoV-2 variant was confirmed by conventional PCR and Sanger sequencing methods.
resultsThe results showed that the melting temperature (T
conclusionsThis study demonstrated a sensitive method that can identify the D614G mutation by a simple two-step RT-qPCR-HRM assay procedure analysis, which can be useful for active surveillance of the transmission of a specific mutation.
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Registered trials
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