Evidence map›Paper›PMID 34513218›Full record

ArticleBiomedical optics express2021

Automated microscope-independent fluorescence-guided micropipette.

Christopher Miranda, Madeleine R Howell, Joel F Lusk, Ethan Marschall, Jarrett Eshima, Trent Anderson, Barbara S Smith

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Christopher MirandaArizona State University, School of Biological and Health Systems Engineering, Tempe, AZ 85210, USA.ORCID https://orcid.org/0000-0002-9996-8550
Madeleine R HowellArizona State University, School of Biological and Health Systems Engineering, Tempe, AZ 85210, USA.
Joel F LuskArizona State University, School of Biological and Health Systems Engineering, Tempe, AZ 85210, USA.
Ethan MarschallArizona State University, School of Biological and Health Systems Engineering, Tempe, AZ 85210, USA.
Jarrett EshimaArizona State University, School of Biological and Health Systems Engineering, Tempe, AZ 85210, USA.
Trent AndersonUniversity of Arizona, College of Medicine - Phoenix, Phoenix, AZ 85004, USA.
Barbara S SmithArizona State University, School of Biological and Health Systems Engineering, Tempe, AZ 85210, USA.ORCID https://orcid.org/0000-0003-1758-624X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Glass micropipette electrodes are commonly used to provide high resolution recordings of neurons. Although it is the gold standard for single cell recordings, it is highly dependent on the skill of the electrophysiologist. Here, we demonstrate a method of guiding micropipette electrodes to neurons by collecting fluorescence at the aperture, using an intra-electrode tapered optical fiber. The use of a tapered fiber for excitation and collection of fluorescence at the micropipette tip couples the feedback mechanism directly to the distance between the target and electrode. In this study, intra-electrode tapered optical fibers provide a targeted robotic approach to labeled neurons that is independent of microscopy.

Identifiers

PMID34513218
PMCPMC8407805

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.