ArticleBiomedical optics express2021
A compact photonic resonator absorption microscope for point of care digital resolution nucleic acid molecular diagnostics.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Ultrasensitive non-enzymatic protein detection using proximity immunoassay with photonic resonator absorption microscopy.Npj biosensing · 2026Article
- Photonic resonator absorption microscopy: why consider metallic and magneto-plasmonic nano-assemblies over bare nanoparticles for digital biosensing?Analytical and bioanalytical chemistry · 2025Article
- Oligonucleotide surface attachment by tosylation for digital detection of microRNA using photonic resonator absorption microscopy.APL bioengineering · 2025Article
- Physically grounded deep learning-enabled gold nanoparticle localization and quantification in photonic resonator absorption microscopy for digital resolution molecular diagnostics.Biosensors & bioelectronics · 2025Article
- Digital Immunoassay for Rapid Detection of SARS-CoV-2 exposure in a Broad Spectrum of Animals.IEEE sensors journal · 2025Article
- Photonic Crystal Enhanced Microscopy on a 2D Photonic Crystal Surface.Advanced materials technologies · 2025Article
- Dynamic and large field of view photonic resonator absorption microscopy for ultrasensitive digital resolution detection of nucleic acid and protein biomarkers.Biosensors & bioelectronics · 2024Article
- Portable, smartphone-linked, and miniaturized photonic resonator absorption microscope (PRAM Mini) for point-of-care diagnostics.Biomedical optics express · 2024Article
- Recent Advances in Research from Nanoparticle to Nano-Assembly: A Review.Nanomaterials (Basel, Switzerland) · 2024Review
- Review of Gold Nanoparticles in Surface Plasmon-Coupled Emission Technology: Effect of Shape, Hollow Nanostructures, Nano-Assembly, Metal-Dielectric and Heterometallic Nanohybrids.Nanomaterials (Basel, Switzerland) · 2024Review
- A photonic resonator interferometric scattering microscope for label-free detection of nanometer-scale objects with digital precision in point-of-use environments.Biosensors & bioelectronics · 2023Article
- A Target Recycling Amplification Process for the Digital Detection of Exosomal MicroRNAs through Photonic Resonator Absorption Microscopy.Angewandte Chemie (International ed. in English) · 2023Article
- Photonic Crystal Enhanced Fluorescence: A Review on Design Strategies and Applications.Micromachines · 2023Review
- Review of HIV Self Testing Technologies and Promising Approaches for the Next Generation.Biosensors · 2023Review
- A Photonic Resonator Interferometric Scattering Microscope for Label-free Detection of Nanometer-Scale Objects with Digital Precision in Point-of-Use Environments.bioRxiv : the preprint server for biology · 2022Article
- Article
- Multiplexed COVID-19 antibody quantification from human sera using label-free nanoplasmonic biosensors.Biomedical optics express · 2022Article
- Microscopies Enabled by Photonic Metamaterials.Sensors (Basel, Switzerland) · 2022Review
- Erratum: A compact photonic resonator absorption microscope for point of care digital resolution nucleic acid molecular diagnostics: publisher's note.Biomedical optics express · 2021Article
- Novel photonic methods for diagnosis of SARS-CoV-2 infection.Translational biophotonicsReview
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
Abstract
Rapid, sensitive, and selective detection of nucleic acid biomarkers for health diagnostic applications becomes feasible for point of care scenarios when the detection instrument is inexpensive, simple, and robust. Here, we report the design, implementation, and characterization of a point of care instrument for photonic resonator absorption microscopy (PRAM) that takes advantage of resonant optical coupling between plasmonic gold nanoparticle tags and a photonic crystal (PC) surface. Matching the PC resonant wavelength to the gold nanoparticle's surface plasmon wavelength generates localized and efficient quenching of the PC resonant reflection intensity, resulting in the ability to clearly detect and count individual gold nanoparticles when they are captured on the PC surface. Surface-captured nanoparticles are observed by illuminating the PC at normal incidence with polarized light from a low-intensity red LED, and recording of PC reflected intensity on an inexpensive CMOS image sensor. A contrast limited adaptive histogram equalization (CLAHE) image processing algorithm was applied to derive counts of captured nanoparticles. The instrument is utilized in the context of an activate capture + digital counting (AC + DC) assay for a specific miRNA sequence, using nucleic acid toehold probes applied to gold nano-urchin (AuNU) nanoparticles to achieve 160 aM detection limits in a 30 min. assay.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.