ArticleJournal of pharmaceutical analysis2021
UHPLC-MS/MS analysis of cAMP and cGMP in rat plasma as potential biomarkers of Yin-Yang disharmony in traditional Chinese medicine.
Article in Journal of pharmaceutical analysis, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed.
- Integrative phytochemical profiling and systems pharmacology identify ERBB2-MAPK1-AKT1 signaling as a molecular basis for the anti-vertigo effects of Ziyin Zhixuan Decoction.Biochemistry and biophysics reports · 2026Article
- Simultaneous spectrofluorimetric determination of nasal cyclic nucleotides as biochemical markers in post-COVID-19 olfactory dysfunction using supramolecular-enhanced derivative spectroscopy.Scientific reports · 2026Article
- Establishment and evaluation of a rat model of cervical spondylosis with Yin deficiency syndrome.American journal of translational research · 2025Article
- The effects of Sijunzi decoction-supplemented cat food on physiological parameters and gut microbiota in healthy adult cats.Frontiers in veterinary science · 2025Article
- The Comprehensive Profiling of the Chemical Components in the Raw and Processed Roots ofMolecules (Basel, Switzerland) · 2024Article
- Ajugol's upregulation of TFEB-mediated autophagy alleviates endoplasmic reticulum stress in chondrocytes and retards osteoarthritis progression in a mouse model.Chinese medicine · 2023Article
- Yin/Yang associated differential responses to Psoralea corylifolia Linn. In rat models: an integrated metabolomics and transcriptomics study.Chinese medicine · 2023Article
- Effects of decoction ofAnnals of translational medicine · 2023Article
- Comparative Pharmacokinetic Study of 5 Active Ingredients after Oral Administration of Zuogui Pill in Osteoporotic Rats with Different Syndrome Types.International journal of analytical chemistry · 2023Article
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6 authors.
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Abstract
Cyclic 3',5'-adenosine monophosphate (cAMP) and cyclic 3',5'-guanosine monophosphate (cGMP) are considered as potential biomarkers for Yin-Yang disharmony in traditional Chinese medicine. However, phosphodiesterase-mediated ex vivo degradation of these molecules in biological samples may result in their underestimation. In the present study, a ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for determination of cAMP and cGMP in rat plasma, with special consideration of their stability ex vivo. Following precipitation of proteins from plasma samples with 0.4 M perchloric acid, the analytes were chromatographed on a Shimadzu Shim-pack-XR-ODS II column with 2.5 mM ammonium acetate and methanol in gradient mode. The MS/MS detection was performed using multiple reaction monitoring in the positive electrospray ionization mode. The lower limit of quantification was 0.27 ng/mL for cAMP and 0.37 ng/mL for cGMP. The method was used to determine the plasma cAMP and cGMP levels in normal and Yin deficiency diabetic rats treated with or without
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