Evidence map›Paper›PMID 34513039›Full record

ArticleSovremennye tekhnologii v meditsine2020

A Novel Method for Amyloid Detection in Human Tissue Load Using a Fluorescent Dye - Congo Red Analogue.

V V Guselnikova, O I Antimonova, E A Fedorova, M M Shavlovsky, A E Safray, A A Rukavishnikova, V V Ilyin, B L Milman, D E Korzhevskii

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Sovremennye tekhnologii v meditsine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 6 institutions in 1 country.

V V GuselnikovaSenior Researcher, Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology, Institute of Experimental Medicine, 12 Akademika Pavlova St., Saint Petersburg, 197376, Russia.
O I AntimonovaJunior Researcher, Laboratory of Human Molecular Genetics, Department of Molecular Genetics, Institute of Experimental Medicine, 12 Akademika Pavlova St., Saint Petersburg, 197376, Russia.
E A FedorovaResearcher, Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology, Institute of Experimental Medicine, 12 Akademika Pavlova St., Saint Petersburg, 197376, Russia.
M M ShavlovskyProfessor, Head of the Laboratory of Human Molecular Genetics, Department of Molecular Genetics, Institute of Experimental Medicine, 12 Akademika Pavlova St., Saint Petersburg, 197376, Russia; Leading Researcher, Cardiomyopathy Laboratory, Institute of Cardiovascular Disease, Pavlov University, 6-8 L'va Tolstogo St., Saint Petersburg, 197022, Russia; Professor, Department of Medical Genetics, North-Western State Medical University named after I.I. Mechnikov, 41 Kirochnaya St., Saint Petersburg, 191015, Russia.
A E SafrayHead of the Forensic Histology Department, The Leningrad Region Bureau of Forensic Medical Expertise, 36-38-40 "B" Shkapina St., 198095, Saint Petersburg, Russia; Lecturer, Department of Forensic Medicine and Law Science, Pavlov University, 6-8 L'va Tolstogo St., Saint Petersburg, 197022, Russia.
A A RukavishnikovaExpert, Forensic Histology Department, The Leningrad Region Bureau of Forensic Medical Expertise, 36-38-40 "B" Shkapina St., 198095, Saint Petersburg, Russia; Lecturer, Department of Forensic Medicine and Law Science, Pavlov University, 6-8 L'va Tolstogo St., Saint Petersburg, 197022, Russia.
V V IlyinResearcher, Laboratory of Synthesis and Nanotechnology of Drugs, S.V. Anichkov Department of Neuropharmacology, Institute of Experimental Medicine, 12 Akademika Pavlova St., Saint Petersburg, 197376, Russia.
B L MilmanHead of the Laboratory of Biomedical and Pharmaceutical Mass-Spectrometry, Institute of Experimental Medicine, 12 Akademika Pavlova St., Saint Petersburg, 197376, Russia.
D E KorzhevskiiProfessor of Russian Academy of Sciences, Head of the Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Institute of Experimental Medicine, 12 Akademika Pavlova St., Saint Petersburg, 197376, Russia; Leading Researcher, Cardiomyopathy Laboratory, Institute of Cardiovascular Disease, Pavlov University, 6-8 L'va Tolstogo St., Saint Petersburg, 197022, Russia.
First Pavlov State Medical University of St. Petersburg · RUInstitute of Experimental Medicine · RUResearch Institute of Human Morphology · RUInstitute of Molecular Genetics · RUNorth-Western State Medical University named after I.I. Mechnikov · RUPavlov Institute of Physiology of the Russian Academy of Sciences · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MATERIALS AND

methodsSynthesis of DSNAF was performed by diazotization of 2,7-diaminofluorene in a stream of argon followed by azo coupling with naphthionic acid. Identification of DSNAF was performed using MALDI mass spectrometry. Human myocardial samples from males and females aged from 85 to 98 years (n=11) were the material for the histochemical study. Myocardial paraffin sections were stained with a 0.1% aqueous solution of Congo red or with an aqueous solution (0.1 or 0.034%) of DSNAF under the same conditions.

resultsIt has been demonstrated for the first time that a new fluorene-based analogue of Congo red, DSNAF, can be successfully used to identify amyloid deposits in histological sections of human myocardium. In terms of the specificity and intensity of amyloid staining, DSNAF is comparable to Congo red, which is the gold standard for detecting amyloid deposits. The fluorescence intensity of DSNAF when binding to amyloid fibrils is significantly higher than the intensity of Congo red fluorescence (with a lower intensity of background fluorescence of heart muscle tissue). This is especially useful for identifying small deposits of amyloid in the human tissues which is important when using small biopsies.

conclusionThe advantages of using DSNAF allow us to consider the developed technology for the detection of amyloid as a new promising method of identifying amyloid deposits in human tissues.

Indexed as

amyloidamyloidosisCongo reddisodium salt of 2,7-(1-amino-4-sulfo-2-naphthylazo)fluorenefluorescence microscopyhuman myocardium

Identifiers

PMID34513039
PMCPMC8353706
OpenAlexW3010196302

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.