Evidence map›Paper›PMID 34512265›Full record

ArticleFrontiers in cellular neuroscience2021

Profiling of Blood-Brain Barrier Disruption in Mouse Intracerebral Hemorrhage Models: Collagenase Injection vs. Autologous Arterial Whole Blood Infusion.

Peijun Jia, Jinxin He, Zefu Li, Junmin Wang, Lin Jia, Ruochen Hao, Jonathan Lai, Weidong Zang, Xuemei Chen, Jian Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular neuroscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 2 pooled it
3.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 2 syntheses or guidelines pooled it, 50 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Peijun JiaDepartment of Anatomy, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Jinxin HeDepartment of Anatomy, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Zefu LiDepartment of Anatomy, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Junmin WangDepartment of Anatomy, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Lin JiaDepartment of Anatomy, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Ruochen HaoDepartment of Anatomy, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Jonathan LaiPre-med Track Majoring in Biology, Baylor University, Waco, TX, United States.
Weidong ZangDepartment of Anatomy, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Xuemei ChenDepartment of Anatomy, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Jian WangDepartment of Anatomy, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Zhengzhou University · CNBaylor University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Disruption of the blood-brain barrier (BBB) and the subsequent formation of brain edema is the most severe consequence of intracerebral hemorrhage (ICH), leading to drastic neuroinflammatory responses and neuronal cell death. A better understanding of ICH pathophysiology to develop effective therapy relies on selecting appropriate animal models. The collagenase injection ICH model and the autologous arterial whole blood infusion ICH model have been developed to investigate the pathophysiology of ICH. However, it remains unclear whether the temporal progression and the underlying mechanism of BBB breakdown are similar between these two ICH models. In this study, we aimed to determine the progression and the mechanism of BBB disruption via the two commonly used murine ICH models: the collagenase-induced ICH model (c-ICH) and the double autologous whole blood ICH model (b-ICH). Intrastriatal injection of 0.05 U collagenase or 20 μL autologous blood was used for a comparable hematoma volume in these two ICH models. Then we analyzed BBB permeability using Evan's blue and IgG extravasation, evaluated tight junction (TJ) damage by transmission electron microscope (TEM) and Western blotting, and assessed matrix metalloproteinase-9 (MMP-9) activity and aquaporin 4 (AQP4) mRNA expression by Gelatin gel zymography and RT-PCR, respectively. The results showed that the BBB leakage was associated with a decrease in TJ protein expression and an increase in MMP-9 activity and AQP4 expression on day 3 in the c-ICH model compared with that on day 5 in the b-ICH model. Additionally, using TEM, we found that the TJ was markedly damaged on day 3 in the c-ICH model compared with that on day 5 in the b-ICH model. In conclusion, the BBB was disrupted in the two ICH models; compared to the b-ICH model, the c-ICH model presented with a more pronounced disruption of BBB at earlier time points, suggesting that the c-ICH model might be a more suitable model for studying early BBB damage and protection after ICH.

Indexed as

aquaporin 4autologous bloodblood-brain barriercollagenaseintracerebral hemorrhagematrix metalloproteinase-9tight junctiontransmission electron microscope

Identifiers

PMID34512265
PMCPMC8427528
OpenAlexW3196390011

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.