ArticlePlant biotechnology journal2022
ERF9 of Poncirus trifoliata (L.) Raf. undergoes feedback regulation by ethylene and modulates cold tolerance via regulating a glutathione S-transferase U17 gene.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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55 citing papers in PubMed, 147 citations in OpenAlex.
- The transcriptional cascade CiERF023Horticulture research · 2026Article
- Large-scale genomic analysis reveals the origin and evolution of Glutathione S-transferases (GSTs) in plants.BMC genomics · 2026Article
- Plasma Membrane-Localized PtCOR8 Enhances Cold Tolerance inPlants (Basel, Switzerland) · 2026Article
- Phytohormone-Mediated Regulation of Plant Cold Stress Tolerance: Signaling, Hormonal Crosstalk, and Translational Perspectives.International journal of molecular sciences · 2026Review
- Lipoxygenase 2 (LOX2) enhances cold tolerance through modulating jasmonate biosynthesis in Nicotiana tabacum.BMC plant biology · 2026Article
- WRKY27-RAP2.7 Regulatory Module Promotes Cold Tolerance via Modulation of Lignin Biosynthesis and Redox Homeostasis by Regulating Cinnamyl Alcohol Dehydrogenase 7 and Glutathione S-Transferase F6.Plant biotechnology journal · 2026Article
- The Transcription Factors NFYA1 and GBF3 Jointly Regulate CHS2 to Promote Tangeretin Accumulation and Cold Tolerance in Citrus.Plant biotechnology journal · 2026Article
- An ERF Gene DcERF3 of Dendrobium catenatum Improves Salt Tolerance in Arabidopsis.Molecular biotechnology · 2026Article
- ERF3 ofFrontiers in plant science · 2026Article
- Conservation and divergence of abiotic stress-responsive gene co-expression networks in diploid and tetraploid cotton.Frontiers in plant science · 2026Article
- The MdGLK1-MdBZR1 Module Integrates Ethylene and Strigolactone Signals to Regulate Cold Tolerance via a CBF-Dependent Pathway in Apple.Plant biotechnology journal · 2025Article
- OsGSTT3 regulates seed germination by modulating reactive oxygen species homeostasis in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Co-Selection of Low Cadmium Accumulation and High Yield During Tomato Improvement.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Identification of Chalcone Synthase Genes and Their Responses to Salt and Cold Stress inPlants (Basel, Switzerland) · 2025Article
- Establishment and application of high-pressure propagation breeding (HPPB)-mediated genetic transformation system in citrus rootstocks.Plant biotechnology journal · 2025Article
- Characterization of a KANADI-like transcription factor that suppresses pear anthocyanin biosynthesis.Horticulture research · 2025Article
- Plant resistance inducer AMHA enhances antioxidant capacities to promote cold tolerance by regulating the upgrade of glutathione S-transferase in tea plant.Horticulture research · 2025Article
- Article
- PiERF1 regulates cold tolerance in Plumbago indica L. through ethylene signalling.Scientific reports · 2025Article
- PfGSTF2 endows resistance to quizalofop-p-ethyl in Polypogon fugax by GSH conjugation.Plant biotechnology journal · 2025Article
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8 authors at 1 institution in 1 country.
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Abstract
Plant ethylene-responsive factors (ERFs) play essential roles in cold stress response, but the molecular mechanisms underlying this process remain poorly understood. In this study, we characterized PtrERF9 from trifoliate orange (Poncirus trifoliata (L.) Raf.), a cold-hardy plant. PtrERF9 was up-regulated by cold in an ethylene-dependent manner. Overexpression of PtrERF9 conferred prominently enhanced freezing tolerance, which was drastically impaired when PtrERF9 was knocked down by virus-induced gene silencing. Global transcriptome profiling indicated that silencing of PtrERF9 resulted in substantial transcriptional reprogramming of stress-responsive genes involved in different biological processes. PtrERF9 was further verified to directly and specifically bind with the promoters of glutathione S-transferase U17 (PtrGSTU17) and ACC synthase1 (PtrACS1). Consistently, PtrERF9-overexpressing plants had higher levels of PtrGSTU17 transcript and GST activity, but accumulated less ROS, whereas the silenced plants showed the opposite changes. Meanwhile, knockdown of PtrERF9 decreased PtrACS1 expression, ACS activity and ACC content. However, overexpression of PtrERF9 in lemon, a cold-sensitive species, caused negligible alterations of ethylene biosynthesis, which was attributed to perturbed interaction between PtrERF9, along with lemon homologue ClERF9, and the promoter of lemon ACS1 gene (ClACS1) due to mutation of the cis-acting element. Taken together, these results indicate that PtrERF9 acts downstream of ethylene signalling and functions positively in cold tolerance via modulation of ROS homeostasis by regulating PtrGSTU17. In addition, PtrERF9 regulates ethylene biosynthesis by activating PtrACS1 gene, forming a feedback regulation loop to reinforce the transcriptional regulation of its target genes, which may contribute to the elite cold tolerance of Poncirus trifoliata.
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