Evidence map›Paper›PMID 34510276›Full record

ArticleInflammopharmacology2021

Comparative assessment of proliferation and immunomodulatory potential of Hypericum perforatum plant and callus extracts on mesenchymal stem cells derived adipose tissue from multiple sclerosis patients.

Negin Afsharzadeh, Fahimeh Lavi Arab, Mojtaba Sankian, Leila Samiei, Nafiseh Sadat Tabasi, Danial Afsharzadeh, Karim Nikkhah, Mahmoud Mahmoudi

Open access · greenAbstract readComparative Study
PubMed Publisher
In one paragraph

Article in Inflammopharmacology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Role of Medicinal Plants in the Management of Multiple Sclerosis.Current pharmaceutical biotechnology · 2025
    Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Negin AfsharzadehImmunology Research Center, Bu-Ali Research Institute, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Fahimeh Lavi ArabImmunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Mojtaba SankianImmunology Research Center, Bu-Ali Research Institute, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Leila SamieiResearch Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Nafiseh Sadat TabasiImmunology Research Center, Bu-Ali Research Institute, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Danial AfsharzadehUniversity Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
Karim NikkhahDepartment of Neurology, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran.
Mahmoud MahmoudiImmunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. MahmoudiM@mums.ac.ir.ORCID http://orcid.org/0000-0002-3193-5242
Mashhad University of Medical Sciences · IRFerdowsi University of Mashhad · IRUniversity Medical Center Groningen · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMesenchymal stem cells-derived adipose tissue (AT-MSCs) are recognized for the treatment of inflammatory diseases including multiple sclerosis (MS). Hypericum perforatum (HP) is an anti-inflammatory pharmaceutical plant with bioactive compounds. Plant tissue culture is a technique to improve desired pharmacological potential. The aim of this study was to compare the anti-inflammatory and proliferative effects of callus with field-growing plant extracts of HP on AT-MSCs derived from MS patients. MATERIALS AND

methodsAT-MSCs were isolated and characterized. HP callus was prepared and exposure to light spectrum (blue, red, blue-red, and control). Total phenols, flavonoids, and hypericin of HP callus and plant extracts were measured. The effects of HP extracts concentrations on proliferation were evaluated by MTT assay. Co-culture of AT-MSCs: PBMCs were challenged by HP plant and callus extracts, and Tregs percentage was assessed by flow cytometry.

resultsIdentification of MSCs was performed. Data showed that blue light could stimulate total phenols, flavonoids, and hypericin. MTT test demonstrated that plant extract in concentrations (0.03, 1.2, 2.5 and 10 μg/ml) and HP callus extract in 10 μg/ml significantly increased. Both HP extracts lead to an increase in Tregs percentage in all concentrations. In particular, a comparison between HP plant and callus extracts revealed that Tregs enhanced 3-fold more than control groups in the concentration of 10 μg/ml callus.

conclusionsHigh concentrations of HP extracts showed effectiveness on AT-MSCs proliferation and immunomodulatory properties with a certain consequence in callus extract. HP extracts may be considered as supplementary treatments for the patients who receiving MSCs transplantation.

Indexed as

Adipose TissueAdultAnti-Inflammatory AgentsCell ProliferationCoculture TechniquesDose-Response Relationship, DrugFemaleHumansHypericumImmunomodulating AgentsMesenchymal Stem CellsMultiple SclerosisPlant ExtractsAnti-Inflammatory AgentsImmunomodulating AgentsPlant ExtractsCallus extractMesenchymal stem cellMultiple sclerosisSt. John's wort

Identifiers

PMID34510276
OpenAlexW3199607373

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.