Evidence map›Paper›PMID 34506810›Full record

ArticleJournal of microbiological methods2021

RAPD PCR detects co-colonisation of multiple group B streptococcus genotypes: A practical molecular technique for screening multiple colonies.

Ka-Ning To, Oliver Powell, Dorota Jamrozy, Rachel Kopunova, Kyriaki Anastasiadou, Amadou Faal, Ousman Secka, Victoria Chalker, Kirsty Le Doare, Elita Jauneikaite

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of microbiological methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Ka-Ning ToDepartment of Infectious Disease, Imperial College London, Norfolk Place, London W2 1PG, UK; Institute of Infection and Immunity, St George's University of London, Cranmer Terrace, London SW17 0RE, UK. Electronic address: k.to15@imperial.ac.uk.
Oliver PowellMRC Centre for Molecular Bacteriology and Infection, Department of Infectious Disease, Imperial College London, 14 Armstrong Road, London SW7 2DD, UK. Electronic address: o.powell18@imperial.ac.uk.
Dorota JamrozyWellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK. Electronic address: dj9@sanger.ac.uk.
Rachel KopunovaDepartment of Medicine, Imperial College London, Norfolk Place, London W2 1PG, UK. Electronic address: rachel.kopunova17@imperial.ac.uk.
Kyriaki AnastasiadouDepartment of Infectious Disease Epidemiology, School of Public Health, Imperial College London, Norfolk Place, London W2 1PG, UK.
Amadou FaalMicrobiology Laboratories, MRC Unit The Gambia at LSHTM, Fajara, P. O. Box 273, The Gambia. Electronic address: afaal@mrc.gm.
Ousman SeckaMicrobiology Laboratories, MRC Unit The Gambia at LSHTM, Fajara, P. O. Box 273, The Gambia. Electronic address: Ousman.Secka@lshtm.ac.uk.
Victoria ChalkerRespiratory and Vaccine Preventable Bacteria Reference Unit, Public Health England, 61 Colindale, London NW9 5EQ, UK. Electronic address: Vicki.Chalker@phe.gov.uk.
Kirsty Le DoareDepartment of Infectious Disease, Imperial College London, Norfolk Place, London W2 1PG, UK; Institute of Infection and Immunity, St George's University of London, Cranmer Terrace, London SW17 0RE, UK. Electronic address: kiledoar@sgul.ac.uk.
Elita JauneikaiteDepartment of Infectious Disease Epidemiology, School of Public Health, Imperial College London, Norfolk Place, London W2 1PG, UK; NHIR Health Protection Research Unit in Healthcare Associated Infections and Antimicrobial Resistance, Department of Infectious Disease, Imperial College London, Hammersmith Campus, Commonwealth Building, London, W12 0NN, UK. Electronic address: e.jauneikaite@imperial.ac.uk.
Imperial College London · GBMRC Unit the Gambia · GMPublic Health England · GBWellcome Sanger Institute · GB

Funding

Medical Research Council MR/R015600/1Wellcome Trust 104482/Z/14/Z
6 · The paper itself

Abstract

Group B Streptococcus (GBS) is a leading cause of neonatal meningitis, pneumonia, and sepsis. The biggest contributing factor of neonatal infections is due to vertical transmission from maternal colonisation of GBS in the genitourinary tract. Multiple serotype colonisation is often not investigated in epidemiological studies, but it is an important consideration for serotype-based vaccine development and implementation to ensure less abundant serotypes are not under-represented. In this study, we show that RAPD PCR is a quick tool useful in screening the presence of genetically different strains using multiple colony picks from a single patient swab. We observed a maximum of five different GBS strains colonising a single patient at a specific time.

Indexed as

DNA, BacterialFemaleHumansInfantMass ScreeningMilk, HumanNasopharynxPolymerase Chain ReactionPolymorphism, Single NucleotideRandom Amplified Polymorphic DNA TechniqueRectumSerogroupStreptococcal InfectionsStreptococcus agalactiaeVaginaWhole Genome SequencingDNA, BacterialCo-colonisationGenetic diversityGroup B StreptococcusMultiple serotypesRAPD PCRS. agalactiaeScreening

Identifiers

PMID34506810
PMCPMC9395992
OpenAlexW3198346244

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.