Evidence map›Paper›PMID 34506726›Full record

ArticleStem cell reports2021

Defining the nature of human pluripotent stem cell-derived interneurons via single-cell analysis.

Thomas Allison, Justin Langerman, Shan Sabri, Marcos Otero-Garcia, Andrew Lund, John Huang, Xiaofei Wei, Ranmal A Samarasinghe, Damon Polioudakis, Istvan Mody and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 28 citations in OpenAlex.

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  4. bioRxiv : the preprint server for biology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

Thomas AllisonDepartment of Biological Chemistry, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.
Justin LangermanDepartment of Biological Chemistry, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.
Shan SabriDepartment of Biological Chemistry, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA; Bioinformatics PhD Program, UCLA, Los Angeles, CA, USA.
Marcos Otero-GarciaCenter for Autism Research and Treatment, Semel Institute, UCLA, Los Angeles, CA, USA.
Andrew LundDepartment of Molecular Cell and Developmental Biology, UCLA, Los Angeles, CA, USA.
John HuangDepartment of Biological Chemistry, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.
Xiaofei WeiDepartment of Neurology, David Geffen School of Medicine UCLA, Los Angeles, CA, USA.
Ranmal A SamarasingheBroad Stem Cell Center for Regenerative Medicine, UCLA, Los Angeles, CA, USA; Department of Neurobiology, UCLA, Los Angeles, CA, USA; Department of Neurology, David Geffen School of Medicine UCLA, Los Angeles, CA, USA; Intellectual and Developmental Disabilities Research Center, UCLA, Los Angeles, CA, USA.
Damon PolioudakisProgram in Neurogenetics, Department of Neurology and Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.
Istvan ModyDepartment of Neurology, David Geffen School of Medicine UCLA, Los Angeles, CA, USA.
Inma CobosDepartment of Pathology, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.
Bennett G NovitchBroad Stem Cell Center for Regenerative Medicine, UCLA, Los Angeles, CA, USA; Department of Neurobiology, UCLA, Los Angeles, CA, USA; Intellectual and Developmental Disabilities Research Center, UCLA, Los Angeles, CA, USA.
Daniel H GeschwindProgram in Neurogenetics, Department of Neurology and Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA; Center for Autism Research and Treatment, Semel Institute, UCLA, Los Angeles, CA, USA; Intellectual and Developmental Disabilities Research Center, UCLA, Los Angeles, CA, USA.
Kathrin PlathBroad Stem Cell Center for Regenerative Medicine, UCLA, Los Angeles, CA, USA; Molecular Biology Institute, UCLA, Los Angeles, CA, USA; Department of Biological Chemistry, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA; Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA; Bioinformatics PhD Program, UCLA, Los Angeles, CA, USA. Electronic address: kplath@mednet.ucla.edu.
William E LowryDepartment of Molecular Cell and Developmental Biology, UCLA, Los Angeles, CA, USA; Broad Stem Cell Center for Regenerative Medicine, UCLA, Los Angeles, CA, USA; Molecular Biology Institute, UCLA, Los Angeles, CA, USA; Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA; Intellectual and Developmental Disabilities Research Center, UCLA, Los Angeles, CA, USA. Electronic address: blowry@ucla.edu.
University of California, Los Angeles · USBroad Center · USCenter for Autism and Related Disorders · US

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Samuel Wheeler French · 1985 to 2026
$134.5M
Roles of sumoylation and alternative polyadenylation in cellular reprogrammingP01GM099134 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PLATH, KATHRIN · 2011 to 2021
$20.3M
TUMOR CELL SURFACES AND CHROMOSOMEST32CA009056 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Gay M Crooks, David A. Nathanson · 1985 to 2026
$10.5M
UCLA IDDRC: Translational CoreP50HD103557 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Peyman Golshani, SUMA JACOB · 2020 to 2026
$9.6M
Integrative Modeling of HIV-Associated Neurocognitive Disorder in Human Brain OrganoidsR01DA051897 · NIDA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI FREGOSO, OLIVER I, NOVITCH, BENNETT G · 2020 to 2024
$3.1M
Translational Neuroscience Training GrantR25NS065723 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CARMICHAEL, STANLEY THOMAS · 2009 to 2023
$2.8M
Regulation of neural progenitor functions underlying cortical growth & complexityR01NS089817 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI NOVITCH, BENNETT G · 2015 to 2019
$2.1M
Resolving selective vulnerability and disease progression in human Alzheimer's brain via single-cell RNA-seqR01AG059848 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI COBOS, INMA · 2018 to 2022
$2.0M
iPS-Glial Restricted Progenitors in White Matter Repair for StrokeR01NS103788 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CARMICHAEL, STANLEY THOMAS, LOWRY, WILLIAM E · 2018 to 2022
$1.6M
Utilizing Human Brain Organoids to Model the Differential Effects of SCN8A Mutation on Cortex and HippocampusK08NS119747 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SAMARASINGHE, RANMAL A · 2021 to 2025
$1.1M
Howard Hughes Medical InstituteNCI NIH HHS P30 CA016042NCI NIH HHS T32 CA009056NIA NIH HHS R01 AG059848NICHD NIH HHS P50 HD103557NIDA NIH HHS R01 DA051897NIGMS NIH HHS P01 GM099134NINDS NIH HHS K08 NS119747NINDS NIH HHS R01 NS089817NINDS NIH HHS R01 NS103788NINDS NIH HHS R25 NS065723
6 · The paper itself

Abstract

The specification of inhibitory neurons has been described for the mouse and human brain, and many studies have shown that pluripotent stem cells (PSCs) can be used to create interneurons in vitro. It is unclear whether in vitro methods to produce human interneurons generate all the subtypes found in brain, and how similar in vitro and in vivo interneurons are. We applied single-nuclei and single-cell transcriptomics to model interneuron development from human cortex and interneurons derived from PSCs. We provide a direct comparison of various in vitro interneuron derivation methods to determine the homogeneity achieved. We find that PSC-derived interneurons capture stages of development prior to mid-gestation, and represent a minority of potential subtypes found in brain. Comparison with those found in fetal or adult brain highlighted decreased expression of synapse-related genes. These analyses highlight the potential to tailor the method of generation to drive formation of particular subtypes.

Indexed as

TranscriptomeCell DifferentiationCellular Reprogramming TechniquesHumansInterneuronsNeural Stem CellsPluripotent Stem CellsSingle-Cell AnalysisTranscription FactorsTranscription Factorshuman brain interneuronneuronal specificationpluripotent stem cellsingle nuclei transcriptomicstranscriptional factor programming

Identifiers

PMID34506726
PMCPMC8514853
OpenAlexW3198477093

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.