Evidence map›Paper›PMID 34505267›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Bacterial Nanocellulose-Based Grafts for Cell Colonization Studies: An In Vitro Bioreactor Perfusion Model.

Max Wacker, Jan Riedel, Priya Veluswamy, Maximilian Scherner, Jens Wippermann, Heike Walles, Jörn Hülsmann

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Max Wacker *Department of Cardiothoracic Surgery, University Hospital Magdeburg, Magdeburg, Germany. max.wacker@med.ovgu.de.
Jan Riedel *Department of Cardiothoracic Surgery, University Hospital Magdeburg, Magdeburg, Germany.
Priya VeluswamyDepartment of Cardiothoracic Surgery, University Hospital Magdeburg, Magdeburg, Germany.
Maximilian SchernerDepartment of Cardiothoracic Surgery, University Hospital Magdeburg, Magdeburg, Germany.
Jens WippermannDepartment of Cardiothoracic Surgery, University Hospital Magdeburg, Magdeburg, Germany.
Heike WallesCore Facility Tissue Engineering, Otto-von-Guericke University Magdeburg, Magdeburg, Germany.
Jörn HülsmannDepartment of Cardiothoracic Surgery, University Hospital Magdeburg, Magdeburg, Germany.
University Hospital Magdeburg · DEOtto-von-Guericke University Magdeburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

With the aging population, the demand for artificial small diameter vascular grafts is constantly increasing, as the availability of autologous grafts is limited due to vascular diseases. A confluent lining with endothelial cells is considered to be a cornerstone for long-term patency of artificial small diameter grafts. We use bacterial nanocellulose off-the-shelf grafts and describe a detailed methodology to study the ability of these grafts to re-colonize with endothelial cells in an in vitro bioreactor model. The viability of the constructs generated in this process was investigated using established cell culture and tissue engineering methods, which includes WST-1 proliferation assay, AcLDL uptake assay, lactate balancing and histological characterization. The data generated this straight forward methodology allow an initial assessment of the principal prospects of success in forming a stable endothelium in artificial vascular prostheses.

Indexed as

BioreactorsEndothelial CellsBlood Vessel ProsthesisPerfusionTissue EngineeringBacterial celluloseCell seedingEndothelializationPerfusion bioreactorSmall diameter vascular graftsVascular tissue engineering

Identifiers

PMID34505267
OpenAlexW3198062834

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.