Evidence map›Paper›PMID 34505216›Full record

ArticleInternational microbiology : the official journal of the Spanish Society for Microbiology2022

Sustained release varnish containing chlorhexidine for prevention of Streptococcus mutans biofilm formation on voice prosthesis surface: an in vitro study.

Menachem Gross, Fadi Ashqar, Ronit Vogt Sionov, Michael Friedman, Ron Eliashar, Batya Zaks, Irith Gati, Danielle Duanis-Assaf, Mark Feldman, Doron Steinberg

Abstract read
PubMed Publisher
In one paragraph

Article in International microbiology : the official journal of the Spanish Society for Microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Menachem GrossDepartment of Otolaryngology - Head and Neck Surgery, Hadassah Hebrew University Medical Center, P.O.Box 12000, 91120, Jerusalem, Israel. drgrossm@hotmail.com.ORCID http://orcid.org/0000-0002-1023-341X
Fadi AshqarDepartment of Otolaryngology - Head and Neck Surgery, Hadassah Hebrew University Medical Center, P.O.Box 12000, 91120, Jerusalem, Israel.ORCID http://orcid.org/0000-0003-3477-6256
Ronit Vogt SionovBiofilm Research Laboratory, Institute of Dental Sciences, Hebrew University, 91120, Jerusalem, Israel.
Michael FriedmanSchool of Medicine, Hebrew University, Jerusalem, Israel.ORCID http://orcid.org/0000-0001-6595-8779
Ron EliasharDepartment of Otolaryngology - Head and Neck Surgery, Hadassah Hebrew University Medical Center, P.O.Box 12000, 91120, Jerusalem, Israel.ORCID http://orcid.org/0000-0003-0740-4975
Batya ZaksBiofilm Research Laboratory, Institute of Dental Sciences, Hebrew University, 91120, Jerusalem, Israel.ORCID http://orcid.org/0000-0001-8123-2302
Irith GatiSchool of Pharmacy, Hebrew University, Jerusalem, Israel.ORCID http://orcid.org/0000-0002-2068-1494
Danielle Duanis-AssafBiofilm Research Laboratory, Institute of Dental Sciences, Hebrew University, 91120, Jerusalem, Israel.ORCID http://orcid.org/0000-0003-4352-8131
Mark FeldmanBiofilm Research Laboratory, Institute of Dental Sciences, Hebrew University, 91120, Jerusalem, Israel.ORCID http://orcid.org/0000-0003-2229-6263
Doron SteinbergBiofilm Research Laboratory, Institute of Dental Sciences, Hebrew University, 91120, Jerusalem, Israel.
Hebrew University of Jerusalem · ILHadassah Medical Center · IL

Funding

The Israeli Cancer Association 20180025
6 · The paper itself

Abstract

objectivesIn this study, we aimed to develop a novel, sustained release varnish (SRV) for voice prostheses (VP) releasing chlorhexidine (CHX), for the prevention of biofilm formation caused by the common oral bacteria Streptococcus mutans on VP surfaces.

methodsThis study was performed in an in vitro model as a step towards future in vivo trials. VPs were coated with a SRV containing CHX (SRV-CHX) or SRV alone (placebo-SRV) that were daily exposed to S. mutans. The polymeric materials of SRV were composed of ethylcellulose and PEG-400. Biofilm formation was assessed by DNA quantification (qPCR), crystal violet staining, confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), and kinetics experiments.

resultsThe amount of DNA in the biofilms formed by S. mutans on VP surfaces coated once with SRV-CHX (1.024 ± 0.218 ng DNA/piece) was 58.5 ± 8.8% lower than that of placebo-SRV-coated VPs (2.465 ± 0.198 ng DNA/piece) after a 48-h exposure to S. mutans (p = 0.038). Reduced biofilm mass on SRV-CHX-coated VPs was visually confirmed by CLSM and SEM. CV staining of SRV-CHX single-coated VPs that have been exposed to S. mutans nine times showed a 98.1 ± 0.2% reduction in biofilm mass compared to placebo-SRV-coated VPs (p = 0.003). Kinetic experiments revealed that SRV-CHX triple-coated VPs could delay bacterial growth for 23 days.

conclusionsCoating VPs with SRV-CHX has an inhibitory effect on biofilm formation and prevents bacterial growth in their vicinities. This study is a proof-of-principle that paves the way for developing new clinical means for reducing both VPs' bacterial biofilm formation and device failure.

Indexed as

Larynx, ArtificialStreptococcus mutansBiofilmsChlorhexidineDelayed-Action PreparationsChlorhexidineDelayed-Action PreparationsBiofilmChlorhexidineLaryngectomySustained release varnishVoice prosthesis

Identifiers

PMID34505216
OpenAlexW3197347600

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.