Evidence map›Paper›PMID 34505088›Full record

ArticleSTAR protocols2021

Intraspinal injection of adeno-associated viruses into the adult mouse spinal cord.

Shrivas Chaterji, Arnab Barik, Anupama Sathyamurthy

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.9field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Shrivas ChaterjiCentre for Neuroscience, Indian Institute of Science, Bangalore, Karnataka 560012, India.
Arnab BarikCentre for Neuroscience, Indian Institute of Science, Bangalore, Karnataka 560012, India.
Anupama SathyamurthyCentre for Neuroscience, Indian Institute of Science, Bangalore, Karnataka 560012, India.
Indian Institute of Science Bangalore · IN

Funding

Wellcome Trust
6 · The paper itself

Abstract

Genetic dissection of neural circuits has been accelerated by recent advances in viral-based vectors. This protocol describes an effective approach to performing intraspinal injections of adeno-associated viruses, which can be used to label, manipulate, and monitor spinal and supraspinal neurons. By avoiding invasive laminectomies and restrictive spinal-clamping and by adopting injectable anaesthetics and tough quartz glass micropipettes, our protocol presents a time-saving and efficient approach for genetic manipulation of neural circuits nucleated in the spinal cord. For complete details on the use and execution of this protocol, please refer to Sathyamurthy et al. (2020).

Indexed as

DependovirusAnimalsGenetic TherapyGenetic VectorsInjections, SpinalMiceSpinal CordMicroscopyModel organismsNeuroscience

Identifiers

PMID34505088
PMCPMC8414904
OpenAlexW3198252209

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.