ArticleSTAR protocols2021
Intrinsic optical signal imaging and targeted injections through a chronic cranial window of a head-fixed mouse.
Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Protocol for recording visual maps in the mouse superior colliculus and visual cortex with intrinsic optical imaging.STAR protocols · 2026Article
- Neurogliaform Cells Exhibit Laminar-specific Responses in the Visual Cortex and Modulate Behavioral State-dependent Cortical Activity.Research square · 2024Article
- Neurogliaform Cells Exhibit Laminar-specific Responses in the Visual Cortex and Modulate Behavioral State-dependent Cortical Activity.bioRxiv : the preprint server for biology · 2024Article
- High-Sensitivity Intrinsic Optical Signal Imaging Through Flexible, Low-Cost Adaptations of an Upright Microscope.eNeuro · 2023Article
- Intrinsic, widefield optical imaging of hemodynamics in rodent models of Alzheimer's disease and neurological injury.Neurophotonics · 2023Review
- Cranial and Spinal Window Preparation forExperimental neurobiology · 2022Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intrinsic optical signal imaging (ISI) is a hemodynamic response-based technique to map the functional architecture of the cortex. ISI is often used as an auxiliary method to localize cortical areas for targeted electrophysiology, pharmacology, or imaging experiments. Here, we provide a protocol for ISI through a cranial window with an access port to identify the area of the primary visual cortex (V1) in a head-fixed mouse, followed by targeted viral vector injection, which enables subsequent two-photon imaging of V1 layer 6 corticothalamic neurons. For complete details on the use and execution of this protocol, please refer to our paper Augustinaite and Kuhn (2020b).
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Registered trials
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