ArticleNPJ biofilms and microbiomes2021
Saliva-derived microcosm biofilms grown on different oral surfaces in vitro.
Article in NPJ biofilms and microbiomes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Comparison of oral collection methods for 16S rRNA gene and shotgun metagenomic sequencing.Microbiology spectrum · 2026Article
- Laminin functionalization of zirconia abutments modulates early oral biofilm formation in vitro.Journal of oral microbiology · 2026Article
- The Respiratory Tract Microbiome and Human Health.Microbial biotechnology · 2025Review
- An in vitro model demonstrating homeostatic interactions between reconstructed human gingiva and a saliva-derived multispecies biofilm.Microbiome · 2025Article
- Advances in modeling periodontal host-microbe interactions: insights from organotypic and organ-on-chip systems.Lab on a chip · 2025Review
- Streptococcus cristatus reduces cariogenicity of saliva-derived microcosms under pH-dependent conditions.Journal of oral microbiology · 2025Article
- Investigation of a novel biofilm model close to the original oral microbiome.Applied microbiology and biotechnology · 2024Article
- Construction of a versatile in vitro cultivation screening platform using human oral microbiota.Environmental microbiology reports · 2024Article
- Article
- Translational challenges and opportunities in biofilm science: a BRIEF for the future.NPJ biofilms and microbiomes · 2022Review
- DEAE-Dextran Coated AgNPs: A Highly Blendable Nanofiller Enhances Compressive Strength of Dental Resin Composites.Polymers · 2022Article
- The Impact of Early Saliva Interaction on Dental Implants and Biomaterials for Oral Regeneration: An Overview.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The microbial composition of a specific oral niche could be influenced by initial bacterial adherence, nutrient and physiological property of the local surface. To investigate the influence of nutrient and surface properties on microbial composition, saliva-derived biofilms were grown in agar on three substrata: Reconstructed Human Gingiva (RHG), a hydroxyapatite (HAP) surface, and a titanium (TI) surface. Agar was mixed with either Brain Heart Infusion (BHI) or Thompson (TP) medium. After 1, 3, or 5 days, biofilm viability (by colony forming units) and microbiome profiles (by 16 S rDNA amplicon sequencing) were determined. On RHG, biofilm viability and composition were similar between BHI and TP. However, on the abiotic substrata, biofilm properties greatly depended on the type of medium and substratum. In BHI, the viability of HAP-biofilm first decreased and then increased, whereas that of TI-biofilm decreased in time until a 6-log reduction. In TP, either no or a 2-log reduction in viability was observed for HAP- or TI-biofilms respectively. Furthermore, different bacterial genera (or higher level) were differentially abundant in the biofilms on 3 substrata: Haemophilus and Porphyromonas for RHG; Bacilli for HAP and Prevotella for TI. In conclusion, RHG, the biotic substratum, is able to support a highly viable and diverse microbiome. In contrast, the viability and diversity of the biofilms on the abiotic substrata were influenced by the substrata type, pH of the environment and the richness of the growth media. These results suggest that the host (oral mucosa) plays a vital role in the oral ecology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.