ArticleNature communications2021
Digital immunoassay for biomarker concentration quantification using solid-state nanopores.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 104 citations in OpenAlex.
- Identification and Discrimination of Sulfated and Unsulfated Gut-Hormone Biomarkers in Human Serum by Nanopore.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Microfluidic Sample Compartmentalization for Biomolecular Concentration Quantification Using a Nanopore Sensor.Small methods · 2026Article
- Catch-and-display immunoassay for digital biomarker detection.Nanoscale horizons · 2026Article
- Research progress and application of solid-state nanopore fabrication based on wet etching.Fundamental research · 2026Review
- Modular RNA:DNA Nanostructures Enable Nanopore Profiling of rRNA Processing and rRNA Variants.ACS nano · 2026Article
- Digital Immunoassays for Sensitive Quantification of Blood Biomarkers Using Solid-State Nanopores.ACS nano · 2026Article
- Tri-Layer Solid-State Nanopore Arrays with Crosstalk Suppression for High-Throughput, Femtomolar-Level Biosensing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A general strategy to enhance aptamer affinity by suppressing dissociation through symmetric assembly.Nucleic acids research · 2026Article
- Catalytic Proximal Protein Oligomerization as an Anti-Tumor Strategy Targeting WDR5.Nature communications · 2026Article
- Catch-and-Display Immunoassay as an Accessible Platform for Digital Biomarker Detection.bioRxiv : the preprint server for biology · 2026Article
- Selective and Controllable Trapping of Single Proteins in Nanopores Using Reversible Covalent Bonds.ACS nano · 2026Article
- Advances in nanomaterial-enhanced immunosensors for ultra-sensitive tumor marker detection: Enabling early cancer diagnostics.Frontiers in chemistry · 2026Review
- Channel-Free Micro-Well-Template-Assisted Magnetic Particle Trapping for Efficient Single-Particle Isolation.Micromachines · 2025Article
- An Automated Micro-Immunobeads-Based Electromagnetic Operation System (MEMOs) for Blood Testing of Alzheimer's Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Single-molecule nanopore sensing of prolineChemical science · 2025Article
- Detection of protein oligomers with nanopores.Nature reviews. Chemistry · 2025Review
- Sub-5 nm Silicon Nanopore Sensors: Scalable Fabrication via Self-Limiting Metal-Assisted Chemical Etching.ACS applied materials & interfaces · 2025Article
- Identification and Detection of a Peptide Biomarker and Its Enantiomer by Nanopore.ACS central science · 2024Article
- Nanopore Translocation Reveals Electrophoretic Force on Noncanonical RNA:DNA Double Helix.ACS nano · 2024Article
- Microfluidic Chip-Assisted Upconversion Luminescence Biosensing Platform for Point-of-Care Virus Diagnostics.Advanced healthcare materials · 2024Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
Abstract
Single-molecule counting is the most accurate and precise method for determining the concentration of a biomarker in solution and is leading to the emergence of digital diagnostic platforms enabling precision medicine. In principle, solid-state nanopores-fully electronic sensors with single-molecule sensitivity-are well suited to the task. Here we present a digital immunoassay scheme capable of reliably quantifying the concentration of a target protein in complex biofluids that overcomes specificity, sensitivity, and consistency challenges associated with the use of solid-state nanopores for protein sensing. This is achieved by employing easily-identifiable DNA nanostructures as proxies for the presence ("1") or absence ("0") of the target protein captured via a magnetic bead-based sandwich immunoassay. As a proof-of-concept, we demonstrate quantification of the concentration of thyroid-stimulating hormone from human serum samples down to the high femtomolar range. Further optimization to the method will push sensitivity and dynamic range, allowing for development of precision diagnostic tools compatible with point-of-care format.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.