ArticleProceedings of the National Academy of Sciences of the United States of America2021
Structural changes in bacteriophage T7 upon receptor-induced genome ejection.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 66 citations in OpenAlex.
- Insights into Genome Ejection by a Therapeutic phiKMV-like Bacteriophage.Journal of molecular biology · 2026Article
- Intact architectures of myophage phi92 in extended and contracted states.PLoS pathogens · 2026Article
- Structural choreography of bacteriophage N4 ejection proteins and the giant virion-associated RNA polymerase.Nature communications · 2026Article
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- Structure of a Brochothrix thermosphacta bacteriophage reveals cell wall adsorption mechanism in Gram-positive infecting siphophages.Nature communications · 2026Article
- Structure of defense against restriction proteins DarA and Hdf in phage P1 reveals a new molecular mechanism during phage assembly, infection and DNA ejection.PLoS pathogens · 2026Article
- Microgravity reshapes bacteriophage-host coevolution aboard the International Space Station.PLoS biology · 2026Article
- The N-Terminal Domain of Tailspike Depolymerases Affects the Replication Efficiency of SyntheticInternational journal of molecular sciences · 2025Article
- High-resolution Cryo-EM Analysis of the Therapeutic Pseudomonas Phage Pa223.Journal of molecular biology · 2025Article
- Structure of the giant RNA polymerase ejected from coliphage N4.Research square · 2025Article
- Targeting multidrug-resistant bacteria with genetic-information-free protein-only phages.bioRxiv : the preprint server for biology · 2025Article
- Replacement of the Genomic Scaffold Improves the Replication Efficiency of SyntheticInternational journal of molecular sciences · 2025Article
- RBPseg: Toward a complete phage tail fiber structure atlas.Science advances · 2025Article
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- Cryo-EM structure of cyanopodophage A4 reveals a pentameric pre-ejectosome in the double-stabilized capsid.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
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- Conformational changes in and translocation of small proteins: insights into the ejection mechanism of podophages.Journal of virology · 2025Article
- Moo19 and B2: Structures ofScience advances · 2024Article
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many tailed bacteriophages assemble ejection proteins and a portal-tail complex at a unique vertex of the capsid. The ejection proteins form a transenvelope channel extending the portal-tail channel for the delivery of genomic DNA in cell infection. Here, we report the structure of the mature bacteriophage T7, including the ejection proteins, as well as the structures of the full and empty T7 particles in complex with their cell receptor lipopolysaccharide. Our near-atomic-resolution reconstruction shows that the ejection proteins in the mature T7 assemble into a core, which comprises a fourfold gene product 16 (gp16) ring, an eightfold gp15 ring, and a putative eightfold gp14 ring. The gp15 and gp16 are mainly composed of helix bundles, and gp16 harbors a lytic transglycosylase domain for degrading the bacterial peptidoglycan layer. When interacting with the lipopolysaccharide, the T7 tail nozzle opens. Six copies of gp14 anchor to the tail nozzle, extending the nozzle across the lipopolysaccharide lipid bilayer. The structures of gp15 and gp16 in the mature T7 suggest that they should undergo remarkable conformational changes to form the transenvelope channel. Hydrophobic α-helices were observed in gp16 but not in gp15, suggesting that gp15 forms the channel in the hydrophilic periplasm and gp16 forms the channel in the cytoplasmic membrane.
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