ArticleProceedings of the National Academy of Sciences of the United States of America2021
Engineering hydrogels with homogeneous mechanical properties for controlling stem cell lineage specification.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- S-Nitrosothiols as Thiol-Protecting Groups for Controlled Thiol-Maleimide Crosslinking of Homogeneous Soft Hydrogels.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Cellular Responses to Mechanical Cues Across Scales: From Fundamental Insights to Translational Potential.Advanced healthcare materials · 2026Review
- Structural Features and Mechanical Properties of Hydrogels Based on PVP Copolymers, Obtained in the Presence of a Solvent.Gels (Basel, Switzerland) · 2025Article
- Synthetic thermoresponsive scaffolds for the expansion and differentiation of human pluripotent stem cells into cardiomyocytes.RSC advances · 2025Article
- Hydrogel Network Architecture Design Space: Impact on Mechanical and Viscoelastic Properties.Gels (Basel, Switzerland) · 2025Review
- Mechanochemistry: Fundamental Principles and Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Hydrogels with programmed spatiotemporal mechanical cues for stem cell-assisted bone regeneration.Nature communications · 2025Article
- Diverse reactivity of maleimides in polymer science and beyond.Polymer international · 2025Article
- Non-Invasive Nanometer Resolution Assessment of Cell-Soft Hydrogel System Mechanical Properties by Scanning Ion Conductance Microscopy.International journal of molecular sciences · 2024Article
- Engineering a Hydrazone and Triazole Crosslinked Hydrogel for Extrusion-Based Printing and Cell Delivery.Advanced healthcare materials · 2024Article
- Tough Hydrogels with Different Toughening Mechanisms and Applications.International journal of molecular sciences · 2024Review
- Chemical and Biological Engineering Strategies to Make and Modify Next-Generation Hydrogel Biomaterials.Matter · 2023Article
- Development of cell-laden multimodular Lego-like customizable endometrial tissue assembly for successful tissue regeneration.Biomaterials research · 2023Article
- Polysaccharide-Based Hydrogels and Their Application as Drug Delivery Systems in Cancer Treatment: A Review.Journal of functional biomaterials · 2023Review
- Non-Covalently Associated Streptavidin Multi-Arm Nanohubs Exhibit Mechanical and Thermal Stability in Cross-Linked Protein-Network Materials.Biomacromolecules · 2022Article
- Cell-3D matrix interactions: recent advances and opportunities.Trends in cell biology · 2022Review
- Article
- Significant Interfacial Dielectric Relaxation of Covalently Bonded Ice-Hydrogels.Gels (Basel, Switzerland) · 2022Article
- Gradual Stress-Relaxation of Hydrogel Regulates Cell Spreading.International journal of molecular sciences · 2022Article
- Regulating the Homogeneity of Thiol-Maleimide Michael-Type Addition-Based Hydrogels Using Amino Biomolecules.Gels (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The extracellular matrix (ECM) is mechanically inhomogeneous due to the presence of a wide spectrum of biomacromolecules and hierarchically assembled structures at the nanoscale. Mechanical inhomogeneity can be even more pronounced under pathological conditions due to injury, fibrogenesis, or tumorigenesis. Although considerable progress has been devoted to engineering synthetic hydrogels to mimic the ECM, the effect of the mechanical inhomogeneity of hydrogels has been widely overlooked. Here, we develop a method based on host-guest chemistry to control the homogeneity of maleimide-thiol cross-linked poly(ethylene glycol) hydrogels. We show that mechanical homogeneity plays an important role in controlling the differentiation or stemness maintenance of human embryonic stem cells. Inhomogeneous hydrogels disrupt actin assembly and lead to reduced YAP activation levels, while homogeneous hydrogels promote mechanotransduction. Thus, the method we developed to minimize the mechanical inhomogeneity of hydrogels may have broad applications in cell culture and tissue engineering.
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