Evidence map›Paper›PMID 34502354›Full record

ArticleInternational journal of molecular sciences2021

Porous Biphasic Calcium Phosphate Granules from Oyster Shell Promote the Differentiation of Induced Pluripotent Stem Cells.

Wen-Fu Ho, Mei-Hwa Lee, James L Thomas, Jin-An Li, Shih-Ching Wu, Hsueh-Chuan Hsu, Hung-Yin Lin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Wen-Fu HoDepartment of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan.ORCID 0000-0003-2355-7939
Mei-Hwa LeeDepartment of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan.
James L ThomasDepartment of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA.
Jin-An LiDepartment of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan.
Shih-Ching WuDepartment of Dental Technology and Materials Science, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan.
Hsueh-Chuan HsuDepartment of Dental Technology and Materials Science, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan.
Hung-Yin LinDepartment of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan.
National University of Kaohsiung · TWCentral Taiwan University of Science and Technology · TWI-Shou University · TWUniversity of New Mexico · US

Funding

Ministry of Science and Technology, Taiwan MOST 106-2221-E-390-013-MY3Ministry of Science and Technology, Taiwan MOST 107-2923-M-390-001-MY3Ministry of Science and Technology, Taiwan MOST 108-2923-B-390-001-MY3Ministry of Science and Technology, Taiwan MOST 109-2314-B-390-001-MY3
6 · The paper itself

Abstract

Oyster shells are rich in calcium, and thus, the potential use of waste shells is in the production of calcium phosphate (CaP) minerals for osteopathic biomedical applications, such as scaffolds for bone regeneration. Implanted scaffolds should stimulate the differentiation of induced pluripotent stem cells (iPSCs) into osteoblasts. In this study, oyster shells were used to produce nano-grade hydroxyapatite (HA) powder by the liquid-phase precipitation. Then, biphasic CaP (BCP) bioceramics with two different phase ratios were obtained by the foaming of HA nanopowders and sintering by two different two-stage heat treatment processes. The different sintering conditions yielded differences in structure and morphology of the BCPs, as determined by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Brunauer-Emmett-Teller (BET) surface area analysis. We then set out to determine which of these materials were most biocompatible, by co-culturing with iPSCs and examining the gene expression in molecular pathways involved in self-renewal and differentiation of iPSCs. We found that sintering for a shorter time at higher temperatures gave higher expression levels of markers for proliferation and (early) differentiation of the osteoblast. The differences in biocompatibility may be related to a more hierarchical pore structure (micropores within macropores) obtained with briefer, high-temperature sintering.

Indexed as

AnimalsAnimal ShellsBiocompatible MaterialsBone RegenerationCalcium PhosphatesCell AdhesionCell DifferentiationCeramicsHumansHydroxyapatitesInduced Pluripotent Stem CellsOsteoblastsOsteogenesisOstreidaePorosityTissue EngineeringBiocompatible Materialscalcium phosphateCalcium Phosphateshydroxyapatite-beta tricalcium phosphateHydroxyapatitesbiocompatibilitybiphasic CaP bioceramicsinduced pluripotent stem cellsoyster shellporous hydroxyapatite granules

Identifiers

PMID34502354
PMCPMC8430496
OpenAlexW3197392982

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.