ArticleJournal of clinical medicine2021
Biological Characterization of Human Autologous Pericardium Treated with the Ozaki Procedure for Aortic Valve Reconstruction.
Article in Journal of clinical medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Pregnancy after Ozaki aortic valve reconstruction in a woman with bicuspid aortic valve: a case report.European heart journal. Case reports · 2026Article
- Natural biomaterials in the management of the aortic valve pathology. Biomedical and clinical aspects: A review.Biomolecules & biomedicine · 2025Review
- Mechanotransduction of autotransplants: remodeling potential of heart valves from autologous pericardial tissue.Frontiers in bioengineering and biotechnology · 2025Review
- Tissue requirements for the application of aortic valve neocuspidization - appropriate pericardium properties and homogeneity?Journal of materials science. Materials in medicine · 2024Article
- Ozaki procedure-re-construction of aortic valve leaflets using autologous pericardial tissue: a review.Indian journal of thoracic and cardiovascular surgery · 2023Review
- The Tricky Connection between Extracellular Vesicles and Mitochondria in Inflammatory-Related Diseases.International journal of molecular sciences · 2023Review
- Progress in the application of patch materials in cardiovascular surgery.Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2023Article
- In Vitro Model for the Evaluation of Innovative Transcatheter Debridement Device (TDD): Pericardium-Based Scaffold and Stem Cells to Reproduce Calcificated Valves.Biomedicines · 2022Article
- Special Issue "New Perspectives in Cardiovascular Surgery".Journal of clinical medicine · 2022Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Ozaki procedure is an innovative surgical technique aiming at reconstructing aortic valves with human autologous pericardium. Even if this procedure is widely used, a comprehensive biological characterization of the glutaraldehyde (GA)-fixed pericardial tissue is still missing.
methodsMorphological analysis was performed to assess the general organization of pericardium subjected to the Ozaki procedure (post-Ozaki) in comparison to native tissue (pre-Ozaki). The effect of GA treatment on cell viability and nuclear morphology was then investigated in whole biopsies and a cytotoxicity assay was executed to assess the biocompatibility of pericardium. Finally, human umbilical vein endothelial cells were seeded on post-Ozaki samples to evaluate the influence of GA in modulating the endothelialization ability in vitro and the production of pro-inflammatory mediators.
resultsThe Ozaki procedure alters the arrangement of collagen and elastic fibers in the extracellular matrix and results in a significant reduction in cell viability compared to native tissue. GA treatment, however, is not cytotoxic to murine fibroblasts as compared to a commercially available bovine pericardium membrane. In addition, in in vitro experiments of endothelial cell adhesion, no difference in the inflammatory mediators with respect to the commercial patch was found.
conclusionsThe Ozaki procedure, despite alteration of ECM organization and cell devitalization, allows for the establishment of a noncytotoxic environment in which endothelial cell repopulation occurs.
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